School of life Sciences, Ulsan National Institute of Science and Technology, 50, UNIST-gil, Ulju-gun 44919, Ulsan, Republic of Korea.
Biofabrication. 2020 Jan 31;12(2):025003. doi: 10.1088/1758-5090/ab5d80.
Recently, decellularized extracellular matrix-based bio-ink (dECM bio-ink) derived from animal organs is attracting attention because of its excellent biocompatibility. However, its poor 3D printability and weak mechanical properties remain a challenge. Here, we developed a new dECM bio-ink with enhanced 3D printability and mechanical properties. dECM micro-particles of about 13.4 μm in size were prepared by decellularizing a porcine liver followed by freeze-milling. The new bio-ink, named as dECM powder-based bio-ink (dECM pBio-ink), was prepared by loading the dECM micro-particles into a gelatin mixture. The usefulness of the dECM pBio-ink was evaluated by assessing its mechanical properties, printability, and cytocompatibility. The results showed that its mechanical properties and 3D printability were greatly improved. Its elastic modulus increased by up to 9.17 times that of the conventional dECM bio-ink. Micro-patterns with living cells were successfully achieved with 93% cell viability. Above all, the new bio-ink showed superior performance in stacking of layers for 3D printing, whereas the conventional bio-ink could not maintain its shape. Finally, we demonstrated that the dECM pBio-ink possessed comparable cytocompatibility with the conventional dECM bio-ink through in vitro tests with endothelial cells and primary mouse hepatocytes.
最近，从动物器官中提取的去细胞外基质生物墨水（dECM 生物墨水）由于其出色的生物相容性而受到关注。然而，其较差的 3D 可打印性和较弱的机械性能仍然是一个挑战。在这里，我们开发了一种新的 dECM 生物墨水，具有增强的 3D 可打印性和机械性能。通过对猪肝脏进行去细胞化，然后进行冷冻粉碎，制备出大小约为 13.4 μm 的 dECM 微球。将这种新的生物墨水命名为基于 dECM 微球的生物墨水（dECM pBio-ink），通过将 dECM 微球载入明胶混合物中制备得到。通过评估其机械性能、可打印性和细胞相容性来评估 dECM pBio-ink 的有用性。结果表明，其机械性能和 3D 可打印性得到了极大的提高。其弹性模量提高了 9.17 倍，最高可达传统的 dECM 生物墨水。用活细胞成功地实现了微图案，细胞存活率高达 93%。最重要的是，新的生物墨水在 3D 打印的层叠方面表现出了卓越的性能，而传统的生物墨水则无法保持其形状。最后，通过内皮细胞和原代小鼠肝细胞的体外试验，我们证明了 dECM pBio-ink 具有与传统 dECM 生物墨水相当的细胞相容性。
