a Research Institute , T&R Biofab Co. Ltd. , Siheung-si , Republic of Korea.
b Department of Advanced Toxicology Research , Korea Institute of Toxicology (KIT) , Daejeon , Republic of Korea.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):644-649. doi: 10.1080/21691401.2019.1575842.
Upon bioprinting, cells are mixed with a biomaterial to fabricate a living tissue, thus emphasizing the importance of biomaterials. The biomaterial used in this study was a bio-ink prepared using skin decellularized extracellular matrix (dECM). Skin dECM was extracted by treating the dermis with chemicals and enzymes; the basic structural and functional proteins of the ECM, including collagen, glycosaminoglycans (GAGs), bioreactive materials and growth factors, were preserved, whereas the resident cells that might cause immune rejection or inflammatory responses were removed. The bio-ink based on dECM powder, together with human dermal fibroblasts (HDFs), was loaded into the nozzle of the 3D bioprinter to create the 3D construct. This construct underwent gelation with changing temperature while its shape was maintained for 7 days. The cells showed over 90% viability and proliferation. By analysing the gene expression pattern in the cells of the construct, the skin regenerative mechanism of the bio-ink was verified. Microarray results confirmed that the gene expression related to skin morphology and development had been enhanced because the bioreactive molecules and growth factors, in addition to residual ECM in dECM, provided an optimal condition for the HDFs.
在生物打印过程中，细胞与生物材料混合以制造活体组织，因此生物材料的重要性不言而喻。本研究中使用的生物材料是使用皮肤去细胞化细胞外基质 (dECM) 制备的生物墨水。通过用化学物质和酶处理真皮来提取皮肤 dECM；保留了 ECM 的基本结构和功能蛋白，包括胶原蛋白、糖胺聚糖 (GAG)、生物反应性材料和生长因子，而可能引起免疫排斥或炎症反应的常驻细胞则被去除。基于 dECM 粉末的生物墨水与人类真皮成纤维细胞 (HDF) 一起装入 3D 生物打印机的喷嘴中以创建 3D 结构。该结构在温度变化时发生凝胶化，同时保持其形状 7 天。细胞的活力和增殖率超过 90%。通过分析构建体中细胞的基因表达模式，验证了生物墨水的皮肤再生机制。微阵列结果证实，与皮肤形态和发育相关的基因表达得到了增强，因为生物反应性分子和生长因子以及 dECM 中的残留 ECM 为 HDF 提供了最佳条件。
