Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, USA.
Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, USA; Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
Anim Reprod Sci. 2019 Dec;211:106203. doi: 10.1016/j.anireprosci.2019.106203. Epub 2019 Oct 18.
The objective of this study was to ascertain cellular characteristics and the dynamics of the sperm chromatin proteins protamine 1 (PRM1) and protamine 2 (PRM2) in the sperm of Holstein bulls having a different fertility status. Important sperm variables were analyzed using computer-assisted sperm analysis (CASA). Sperm membrane, acrosome status, DNA integrity were also assessed using propidium iodide (PI), fluorescein isothiocyanate conjugated to Arachis hypogaea (FITC-PNA), and acridine orange (AO) followed by flow cytometry. In addition, abundances of PRM1 and PRM2 were analyzed using flow cytometry experiments. Differences in sperm decondensation capacity were assessed in bulls of varying fertility using a decondensation assay. As determined using CASA, average pathway velocity, amplitude of lateral head displacement and straightness were different (P < 0.05) for sperm from high and low fertility bulls. There, however, were no differences between the high and low fertility bulls for characteristics of sperm plasma membrane, acrosome, and DNA integrity (P > 0.05). Relative abundances of PRM1 and PRM2 in sperm from the high and low fertility bulls were inversely related (P < 0.0001). Percentages of decondensed sperm were different between high and low fertility bulls (P < 0.0001) and total numbers of decondensed sperm were greater in low fertility bulls than high fertility bulls (R = 0.72). Results of the present study are significant because molecular and morphological phenotypes of sperm that were detected affect fertility in livestock species.
本研究旨在确定具有不同生育能力状态的荷斯坦公牛精子中精蛋白 1 (PRM1) 和精蛋白 2 (PRM2) 的细胞特征和精子染色质蛋白动力学。使用计算机辅助精子分析 (CASA) 分析了重要的精子变量。还使用碘化丙啶 (PI)、花生凝集素结合异硫氰酸荧光素 (FITC-PNA) 和吖啶橙 (AO) 评估了精子膜、顶体状态和 DNA 完整性，然后通过流式细胞术进行评估。此外，还使用流式细胞术实验分析了 PRM1 和 PRM2 的丰度。使用解凝聚试验评估了具有不同生育能力的公牛的精子解凝聚能力差异。如 CASA 所确定的，高生育力和低生育力公牛的精子平均路径速度、侧向头部位移幅度和直线度不同（P < 0.05）。然而，高生育力和低生育力公牛的精子质膜、顶体和 DNA 完整性特征没有差异（P > 0.05）。高生育力和低生育力公牛精子中 PRM1 和 PRM2 的相对丰度呈负相关（P < 0.0001）。高生育力和低生育力公牛之间的解凝聚精子百分比不同（P < 0.0001），低生育力公牛的总解凝聚精子数多于高生育力公牛（R = 0.72）。本研究的结果具有重要意义，因为检测到的精子的分子和形态表型会影响家畜的生育能力。
