Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland; Department of Food Hygiene and Control, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.
Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland.
J Glob Antimicrob Resist. 2020 Mar;20:41-42. doi: 10.1016/j.jgar.2019.11.015. Epub 2019 Nov 28.
The aim of this study was to investigate the occurrence of plasmid-encoded colistin resistance among Gram-negative bacteria isolated from meat and meat products in Egypt and to report the draft genome sequence of anmcr-1/IncI2-carrying multidrug-resistant (MDR) Escherichia coli B1:ST101 isolate.
A total of 128 colistin-resistant strains were isolated from various meat and meat product samples in different cities in Egypt. Multiplex PCR screening for plasmid-mediated colistin resistance genes was performed. Whole-genome sequencing was performed using an Illumina NextSeq platform and the genome was assembled using CLC Genomics Workbench 7.5.1.
A singlemcr-1-positive MDR E. coli strain was isolated from beef sausages. The genome size of the E. coli strain was calculated at 5 044 715bp, with a total of 226 contigs and a G+C content of 50.5%. The strain belonged to ST101 (phylogroup B1). The mcr-1 gene was located on an IncI2-type self-conjugative plasmid of 64.6kb in size. The strain showed a MDR phenotype, with a colistin MIC of 4mg/L. A large number of acquired antimicrobial resistance genes was identified, including genes encoding resistance to colistin (mcr-1), β-lactams (bla), phenicols (floR), trimethoprim (dfrA12), aminoglycosides [aac(3)-IIa, aph(3")-Ib and aadA2], macrolides (mphA and mdfA), tetracyclines (tetA), sulfonamides (sul1 and sul2) and quinolones (qnrS1).
Here we report the first draft genome sequence of anmcr-1/IncI2-carrying MDR E. coli B1:ST101 isolated from beef sausage in Egypt. This study highlights the potential role played by food products in the spread of colistin resistance to humans.
本研究旨在调查埃及肉类和肉类产品中分离的革兰氏阴性菌质粒编码的黏菌素耐药情况，并报告携带 anmcr-1/IncI2 的多药耐药（MDR）大肠杆菌 B1:ST101 分离株的基因组序列草案。
从埃及不同城市的各种肉类和肉类产品样本中分离出 128 株黏菌素耐药株。采用多重 PCR 筛选方法检测质粒介导的黏菌素耐药基因。使用 Illumina NextSeq 平台进行全基因组测序，使用 CLC Genomics Workbench 7.5.1 进行基因组组装。
从牛肉香肠中分离出一株单 mcr-1 阳性 MDR 大肠杆菌。该大肠杆菌菌株的基因组大小计算为 5044715bp，共有 226 个 contigs，GC 含量为 50.5%。该菌株属于 ST101（进化群 B1）。mcr-1 基因位于大小为 64.6kb 的 IncI2 型自我接合质粒上。该菌株表现出 MDR 表型，对黏菌素的 MIC 为 4mg/L。鉴定出大量获得性抗菌药物耐药基因，包括编码对黏菌素（mcr-1）、β-内酰胺类（bla）、苯氧乙醇（floR）、三氨嘧啶（dfrA12）、氨基糖苷类 [aac(3)-IIa、aph(3")-Ib 和 aadA2]、大环内酯类（mphA 和 mdfA）、四环素类（tetA）、磺胺类（sul1 和 sul2）和喹诺酮类（qnrS1）耐药的基因。
本研究首次报告了从埃及牛肉香肠中分离出的携带 anmcr-1/IncI2 的 MDR 大肠杆菌 B1:ST101 的基因组序列草案。本研究强调了食品在将黏菌素耐药性传播给人类方面所起的潜在作用。
