Department of Microbiology and Immunology, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt.
Alexandria University Hospital, Alexandria University, Alexandria, Egypt.
Pol J Microbiol. 2022 Mar 30;71(1):83-90. doi: 10.33073/pjm-2022-011.
Antimicrobial resistance represents a global dilemma. Our present study aimed to investigate the presence of among different Gram-negative bacteria including (except intrinsically resistant to colistin) and . Gram-negative bacterial isolates were collected from different ICUs in several Alexandria hospitals from June 2019 to June 2020. The identification of these Gram-negative isolates was made using the VITEK-2 system (BioMérieux, France). SYBR Green-based PCR was used to screen for the presence of using a positive control that we amplified and sequenced earlier in our pilot study. All isolates were screened for the presence of regardless of their colistin susceptibility. Isolates that harbored were tested for colistin susceptibility and for the presence of some beta-lactamase genes. isolates harboring were capsule typed using the sequence analysis. Four hundred eighty isolates were included in this study. Only six isolates harbored . Of these, four were resistant to colistin, while two ( and ) were susceptible to colistin. Five of the six isolates were resistant to carbapenems. They harbored , and three of them co-harbored . K-58 was the most often found among our harboring . To our knowledge, this is the first time to report colistin susceptible and harboring the gene in Egypt. Further studies are needed to investigate the presence of the genes among colistin susceptible isolates to shed more light on its significance as a potential threat.
抗微生物药物耐药性是一个全球性的难题。本研究旨在调查不同革兰氏阴性菌中 （除固有耐药于粘菌素的细菌外） 和 的存在情况。从 2019 年 6 月至 2020 年 6 月，从亚历山大几家医院的不同 ICU 采集革兰氏阴性细菌分离株。使用 VITEK-2 系统（法国生物梅里埃公司）进行这些革兰氏阴性分离物的鉴定。使用 SYBR Green 为基础的 PCR 筛选 基因的存在情况，使用我们在之前的初步研究中扩增和测序的阳性对照进行筛选。无论其对粘菌素的敏感性如何，所有分离株都被筛选出 基因的存在情况。携带 基因的分离株被测试对粘菌素的敏感性以及某些β-内酰胺酶基因的存在情况。使用 序列分析对携带 基因的 分离株进行荚膜分型。本研究共纳入 480 株分离株。仅 6 株分离株携带 基因。其中，4 株对粘菌素耐药，而 2 株（ 和 ）对粘菌素敏感。6 株携带 基因的分离株中有 5 株对碳青霉烯类耐药。它们携带 、 和 ，其中 3 株共同携带 。在我们携带 的分离株中，K-58 是最常见的。据我们所知，这是首次在埃及报告对粘菌素敏感的 和 携带 基因。需要进一步研究以调查对粘菌素敏感的分离株中 基因的存在情况，以更深入地了解其作为潜在威胁的意义。
