非洲猪瘟病毒的冷冻电镜结构。

Cryo-EM Structure of the African Swine Fever Virus.

机构信息

School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

State Key Laboratory of Veterinary Biotechnology and National High-Containment Laboratory for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China.

出版信息

Cell Host Microbe. 2019 Dec 11;26(6):836-843.e3. doi: 10.1016/j.chom.2019.11.004. Epub 2019 Nov 28.

DOI:10.1016/j.chom.2019.11.004

PMID:31787524

Abstract

African swine fever virus (ASFV) is a large double-stranded DNA virus with an icosahedral multilayered structure. ASFV causes a lethal swine hemorrhagic disease and is currently responsible for widespread damage to the pork industry in Asia. Neither vaccines nor antivirals are available and the molecular characterization of the ASFV particle is outstanding. Here, we describe the cryogenic electron microscopy (cryo-EM) structure of the icosahedral capsid of ASFV at 4.6-Å. The ASFV particle consists of 8,280 copies of the major capsid protein p72, 60 copies of the penton protein, and at least 8,340 minor capsid proteins, of which there might be 3 different types. Like other nucleocytoplasmic large DNA viruses, the minor capsid proteins form a hexagonal network below the outer capsid shell, functioning as stabilizers by "gluing" neighboring capsomers together. Our findings provide a comprehensive molecular model of the ASFV capsid architecture that will contribute to the future development of countermeasures, including vaccines.

摘要

非洲猪瘟病毒（ASFV）是一种具有二十面体多层结构的大型双链 DNA 病毒。ASFV 引起致命的猪出血性疾病，目前正导致亚洲猪肉产业遭受广泛破坏。既没有疫苗也没有抗病毒药物，而且 ASFV 粒子的分子特征非常突出。在这里，我们描述了 ASFV 二十面体衣壳的低温电子显微镜（cryo-EM）结构，分辨率为 4.6-Å。ASFV 粒子由 8,280 个主要衣壳蛋白 p72、60 个五邻体蛋白和至少 8,340 个次要衣壳蛋白组成，其中可能有 3 种不同类型。与其他核质大 DNA 病毒一样，次要衣壳蛋白在外壳下方形成六方网络，通过“胶合”相邻的衣壳蛋白起到稳定作用。我们的发现提供了 ASFV 衣壳结构的全面分子模型，这将有助于未来开发对策，包括疫苗。