Kleinwort Kristina J H, Hauck Stefanie M, Degroote Roxane L, Scholz Armin M, Hölzel Christina, Maertlbauer Erwin P, Deeg Cornelia
Chair of Animal Physiology, Department of Veterinary Sciences, LMU Munich, Munich, Germany.
Research Unit for Protein Science, Helmholtz Zentrum Munich, German Research Center for Environmental Health GmbH, Munich, Germany.
PeerJ. 2019 Nov 25;7:e8130. doi: 10.7717/peerj.8130. eCollection 2019.
subsp. (MAP) is a pathogen causing paratuberculosis in cattle and small ruminants. During the long asymptomatic subclinical stage, high numbers of MAP are excreted and can be transmitted to food for human consumption, where they survive many of the standard techniques of food decontamination. Whether MAP is a human pathogen is currently under debate. The aim of this study was a better understanding of the host-pathogen response by analyzing the interaction of peripheral blood lymphocytes (PBL) from cattle with MAP in their exoproteomes/secretomes to gain more information about the pathogenic mechanisms of MAP. Because in other mycobacterial infections, the immune phenotype correlates with susceptibility, we additionally tested the interaction of MAP with recently detected cattle with a different immune capacity referred as immune deviant (ID) cows. In PBL, different biological pathways were enhanced in response to MAP dependent on the immune phenotype of the host. PBL of control cows activated members of cell activation and chemotaxis of leukocytes pathway as well as IL-12 mediated signaling. In contrast, in ID cows CNOT1 was detected as highly abundant protein, pointing to a different immune response, which could be favorable for MAP. Additionally, MAP exoproteomes differed in either GroEL1 or DnaK abundance, depending on the interacting host immune response. These finding point to an interdependent, tightly regulated response of the bovine immune system to MAP and vise versa.
亚种（MAP）是一种导致牛和小型反刍动物患副结核病的病原体。在漫长的无症状亚临床阶段，大量的MAP会被排出体外，并可能传播到供人类食用的食物中，而且它们能在许多标准的食品去污技术下存活下来。MAP是否为人类病原体目前仍存在争议。本研究的目的是通过分析牛外周血淋巴细胞（PBL）与MAP在外蛋白组/分泌蛋白组中的相互作用，更好地了解宿主-病原体反应，以获取更多关于MAP致病机制的信息。由于在其他分枝杆菌感染中，免疫表型与易感性相关，我们还测试了MAP与最近检测到的具有不同免疫能力的牛（称为免疫异常（ID）牛）之间的相互作用。在PBL中，根据宿主的免疫表型，不同的生物学途径会因MAP而增强。对照牛的PBL激活了细胞活化和白细胞趋化途径的成员以及IL-12介导的信号传导。相比之下，在ID牛中，CNOT1被检测为高度丰富的蛋白质，这表明存在不同的免疫反应，这可能对MAP有利。此外，根据相互作用的宿主免疫反应，MAP外蛋白组中GroEL1或DnaK的丰度有所不同。这些发现表明牛免疫系统对MAP的反应是相互依存且受到严格调控的，反之亦然。
