Hao Mingjiao, Wang Jinghui, Zhao Jiadi, Liu Nan, Feng Chi, Wang Ziping, Sun Danhui, Hu Quanli, Wang Zhiyu, Wang Feng, Yang Jingfeng, Lu Luhua, Dong Wu, Duan Limei, Liu Zhengang, Liu Jinghai
Inner Mongolia Key Lab of Carbon Nanomaterials, Nano Innovation Institute (NII), College of Chemistry and Chemical Engineering, Inner Mongolia University for Nationalities (IMUN), Tongliao, Inner Mongolia 028000, P. R. China.
The Quality&Safety Center of Agricultural and Animal Products Ministry of Tongliao, Tongliao, Inner Mongolia 028000, P. R. China.
ACS Omega. 2019 Nov 14;4(22):19655-19663. doi: 10.1021/acsomega.9b02289. eCollection 2019 Nov 26.
Photocatalytic oxidation treatment is an emerging and fast developed eco-friendly, energy-saving, and efficient advanced oxidation technology for degrading hazardous pesticides. The conventional chemical detection to evaluate the effects for this process depends on the broken chemical structure, only giving residual content and product chemical composition. However, it misses direct visual detection on the toxicity and the quantitative analysis of pesticide detoxification. Here, we develop a novel strategy to combine photocatalytic oxidation with a zebrafish biological model to provide a direct visual detection on the environmental detoxification. The mortality or deformity of zebrafish embryos (ZEs) acts as an indicator. Over the irradiation duration threshold, the mortality of ZEs decreases to 23.3% for pure chlorothalonil (CTL-P) after photocatalytic oxidation treatment for 1 h, and the deformity reduces to 13.3% for commercial CTL (CTL-C) after 30 min and to 3.33% for tetramethylthiuram disulfide (TMTD) after 20 min. The toxicity of CTL-C and TMTD could be completely removed by photocatalytic oxidation treatment and causes no damage to the ZE developmental morphology. Chemical analyses demonstrate the degradation of CTL into inorganic compounds and TMTD into small organic molecules. Among these highlighted heterogeneous photocatalysts (g-CN, BiVO, AgPO, and P25), g-CN exhibits the highest photocatalytic detoxification for CTL-P, CTL-C, and TMTD.
光催化氧化处理是一种新兴且快速发展的环保、节能、高效的高级氧化技术，用于降解有害农药。传统的化学检测方法来评估该过程的效果依赖于化学结构的破坏，只能给出残留含量和产物的化学成分。然而，它缺少对毒性的直接可视化检测以及农药解毒的定量分析。在此，我们开发了一种新策略，将光催化氧化与斑马鱼生物模型相结合，以提供对环境解毒的直接可视化检测。斑马鱼胚胎（ZEs）的死亡率或畸形率作为指标。在照射持续时间阈值以上，光催化氧化处理1小时后，纯百菌清（CTL-P）处理的ZEs死亡率降至23.3%，商业百菌清（CTL-C）处理30分钟后畸形率降至13.3%，二硫化四甲基秋兰姆（TMTD）处理20分钟后畸形率降至3.33%。光催化氧化处理可完全去除CTL-C和TMTD的毒性，且对ZE的发育形态无损害。化学分析表明，CTL降解为无机化合物，TMTD降解为小分子有机化合物。在这些突出的非均相光催化剂（g-CN、BiVO、AgPO和P25）中，g-CN对CTL-P、CTL-C和TMTD表现出最高的光催化解毒能力。
