Kakkanaiah Vellalore N, Pan Fan, Bennett Patrick
Biomarker Laboratories, PPD, Richmond, VA 23230, USA.
Bioanalysis. 2019 Oct;11(20):1881-1884. doi: 10.4155/bio-2019-0170.
We evaluated the sample stability for a cellular kinetics and a pharmacodynamic flow cytometry methods. First, the blood collection tubes were compared for the enumeration of chimeric antigen receptor-T cells in human whole blood. Blood samples with chimeric antigen receptor-T cells were stable up to 3 days at room temperature in both conventional EDTA and Cyto-Chex blood collection tubes (Streck Laboratories, NE, USA), but with better consistency in Cyto-Chex-BCT than conventional EDTA tubes. Second, sample storage temperatures were compared for the basophil activation test in human whole blood samples. The samples were stable up to 3 days for basophil activation test when stored at refrigerator temperature, but not stable when stored at room temperature. It is crucial during the development of method to evaluate all the variables which might impact sample integrity.
我们评估了细胞动力学和药效学流式细胞术方法的样本稳定性。首先，比较了用于全血中嵌合抗原受体T细胞计数的采血管。含有嵌合抗原受体T细胞的血样在室温下于传统乙二胺四乙酸（EDTA）管和Cyto-Chex采血管（美国内布拉斯加州Streck实验室）中均可稳定保存3天，但Cyto-Chex-BCT管中的一致性优于传统EDTA管。其次，比较了全血样本中嗜碱性粒细胞活化试验的样本储存温度。样本在冷藏温度下保存时，嗜碱性粒细胞活化试验可稳定3天，但在室温下保存时不稳定。在方法开发过程中，评估所有可能影响样本完整性的变量至关重要。
