Department of Systems Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea.
Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.
Chem Commun (Camb). 2019 Dec 28;55(100):15133-15136. doi: 10.1039/c9cc08503c. Epub 2019 Dec 2.
Herein we report the development of an efficient cellular system for the in vivo biosynthesis of Tyr-analogs and their concurrent incorporation into target proteins by the residue-specific approach. This system makes use of common phenol derivatives and the tyrosine phenol lyase machinery to create various tyrosine analogues that impart desired properties on the target proteins. Biosynthesized 2-fluorotyrosine was incorporated into three industrially important enzymes which resulted in enhanced thermostability.
在这里，我们报告了一种有效的细胞系统的发展，用于体内生物合成 Tyr 类似物，并通过残基特异性方法将其同时掺入靶蛋白中。该系统利用常见的酚衍生物和酪氨酸酚裂解酶机制来创建各种赋予目标蛋白所需特性的酪氨酸类似物。生物合成的 2-氟酪氨酸被掺入三种工业上重要的酶中，从而提高了热稳定性。
