Rundell J O, Evans C H
Immunopharmacology. 1979 Dec;2(1):19-27. doi: 10.1016/0162-3109(79)90016-x.
The generation of serum-free lymphotoxin by antigen-stimulated ovalbumin immune strain 2/N guinea pig peritoneal leukocytes in vitro is dependent upon a number of common variables in cell culture. Lymphotoxin production varies directly with antigen concentration, duration of antigen exposure and leukocyte culture, leukocyte density, and medium volume. The maximum concentration of lymphotoxin generated in the medium has a limiting value that is independent of antigen concentration, leukocyte density, and duration of antigen exposure or leukocyte culture, suggesting feedback control of lymphokine production. Optimal conditions for the reproducible maximum generation of guinea pig lymphotoxin were 10(7) oil-induced peritoneal leukocytes from immune animals cultured for 12-24 hr in 20 ml RPMI 1640 medium containing 100 micrograms ovalbumin/ml throughout the period of culture.
抗原刺激的卵清蛋白免疫的2/N品系豚鼠腹膜白细胞在体外产生无血清淋巴毒素取决于细胞培养中的一些常见变量。淋巴毒素的产生与抗原浓度、抗原暴露时间和白细胞培养时间、白细胞密度以及培养基体积直接相关。培养基中产生的淋巴毒素的最大浓度有一个极限值，该值与抗原浓度、白细胞密度、抗原暴露时间或白细胞培养时间无关，提示存在淋巴因子产生的反馈控制。产生可重复的最大量豚鼠淋巴毒素的最佳条件是，在整个培养期间，将来自免疫动物的10⁷个经油诱导的腹膜白细胞，在含有100微克/毫升卵清蛋白的20毫升RPMI 1640培养基中培养12 - 24小时。
