Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman 7616914111, Iran.
Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman 7616914111, Iran; Research and Technology Institute of Plant Production (RTIPP), Shahid Bahonar University of Kerman, 7616914111, Iran.
Virus Res. 2020 Jan 15;276:197830. doi: 10.1016/j.virusres.2019.197830. Epub 2019 Nov 29.
Members of the family Nanoviridae are multi-component single-stranded DNA viruses that infect a variety of plant species. Using a combination of conventional PCR and high throughput sequencing-based approach, we identified a novel nanovirus infecting two symptomatic milk vetch plants (Astragalus myriacanthus Boiss.; family Fabaceae) showing marginal leaf chlorosis, little leaves and dwarfing in Iran. All eight segments (DNA-C, DNA-M, DNA-N, DNA-R, DNA-S, DNA-U1, DNA-U2 and DNAU4) were recovered and Sanger sequenced. The genome of this new nanovirus, hereby referred to as milk vetch chlorotic dwarf virus (MVCDV), shares 62.2-74.7 % nucleotide pairwise identity with the genomes of other nanoviruses. DNA-C, DNA-M, DNA-N, DNA-S components are most closely related to those of black medic leaf roll virus (BMLRV), sharing between 67.8-81.2 % identity. We also identified three nanoalphasatellites (family Alphasatellitidae) associated with the nanovirus which belong to species Faba bean necrotic yellows alphasatellite 1 (genus Subclovsatellite), Faba bean necrotic yellows alphasatellite 2 (genus Fabenesatellite) and Sophora yellow stunt alphasatellite 5 (genus Clostunsatellite). Given the significant diversity of Astragalus spp. in Iran, it is likely that there could be more nanoviruses circulating in these plants and that these may play a role in the spread of these nanovirus to cultivated fabaceous hosts.
家族 Nanoviridae 的成员是多组分单链 DNA 病毒，感染多种植物物种。我们使用常规 PCR 和高通量测序相结合的方法，鉴定出一种感染两种症状性黄芪植物（Astragalus myriacanthus Boiss.；豆科）的新型纳米病毒，表现为边缘叶褪绿、小叶和矮化。所有八个片段（DNA-C、DNA-M、DNA-N、DNA-R、DNA-S、DNA-U1、DNA-U2 和 DNAU4）均被回收并进行 Sanger 测序。这种新的纳米病毒，暂命名为黄芪褪绿矮化病毒（MVCDV），与其他纳米病毒的基因组具有 62.2-74.7%的核苷酸序列同一性。DNA-C、DNA-M、DNA-N 和 DNA-S 组分与黑 medic 叶卷病毒（BMLRV）最为密切相关，同源性为 67.8-81.2%。我们还鉴定了与纳米病毒相关的三种纳米alphasatellites（Alphasatellitidae 家族），它们属于蚕豆坏死黄化 alphasatellite 1（属 Subclovsatellite）、蚕豆坏死黄化 alphasatellite 2（属 Fabenesatellite）和苦参黄矮 alphasatellite 5（属 Clostunsatellite）。鉴于伊朗黄芪属植物的多样性，可能有更多的纳米病毒在这些植物中循环，并且这些病毒可能在纳米病毒传播到栽培豆科宿主中发挥作用。
