Institute of Fluid Science, Tohoku University, Japan.
Graduate School of Biomedical Engineering, Tohoku University, Japan.
Technol Health Care. 2020;28(4):345-354. doi: 10.3233/THC-191911.
Blood vessels are constantly exposed to flow-induced stresses, and endothelial cells (ECs) respond to these stresses in various ways.
In order to facilitate endothelialization after endovascular implantation, cell behaviors around a metallic wire using a flow circulation system are observed.
A parallel flow chamber was designed to reproduce constant shear stresses (SSs) on cell surfaces and to examine the effects of a straight bare metal wire on cell monolayers. Cells were then exposed to flow for 24 h under SS conditions of 1, 2, and 3 Pa. Subsequently, cell distributions were observed on the plate of the flow chamber and on the surface of the bare metal wire. Flow fields inside the flow chamber were analyzed using computational fluid dynamics under each SS condition.
After 24 h, ECs on the bottom plate were concentrated toward the area of flow reattachment. The matching of higher cell density and CFD result suggests that flow-induced stimuli have an influence on EC distributions.
Typical cell concentration occurs on dish plate along the vortexes, which produces large changes in SSs on cell layer.
血管不断受到流动引起的压力，内皮细胞（ECs）以各种方式对这些压力做出反应。
为了促进血管内植入后的内皮化，使用流动循环系统观察金属丝周围的细胞行为。
设计了一个平行流室，以在细胞表面重现恒定剪切应力（SS），并研究直裸金属丝对细胞单层的影响。然后，在 1、2 和 3 Pa 的 SS 条件下将细胞暴露于流动 24 小时。随后，观察流动室内平板和裸金属丝表面上的细胞分布。在每个 SS 条件下，使用计算流体动力学分析流动室内的流场。
24 小时后，底部平板上的 EC 集中在流动再附着区域。更高的细胞密度与 CFD 结果的匹配表明，流动引起的刺激对 EC 分布有影响。
典型的细胞浓度沿旋涡出现在碟板上，这会导致细胞层上的 SS 发生较大变化。
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