University of Washington, Department of Psychiatry and Behavioral Sciences, Seattle, WA, 98195, USA.
University of Washington, Department of Genome Sciences, Seattle, WA, 98195, USA.
Sci Data. 2019 Dec 3;6(1):303. doi: 10.1038/s41597-019-0308-y.
The heterotrophic marine bacterium, Ruegeria pomeroyi, was experimentally cultured under environmentally realistic carbon conditions and with a tracer-level addition of C-labeled leucine to track bacterial protein biosynthesis through growth phases. A combination of methods allowed observation of real-time bacterial protein production to understand metabolic priorities through the different growth phases. Over 2000 proteins were identified in each experimental culture from exponential and stationary growth phases. Within two hours of the C-labeled leucine addition, R. pomeroyi significantly assimilated the newly encountered substrate into new proteins. This dataset provides a fundamental baseline for understanding growth phase differences in molecular physiology of a cosmopolitan marine bacterium.
实验条件下，异养海洋细菌鲁杰氏菌（Ruegeria pomeroyi）在具有环境现实性的碳条件下，并在痕量添加 C 标记亮氨酸的情况下被培养，以跟踪细菌蛋白质生物合成通过生长阶段。通过结合多种方法，可以观察到实时细菌蛋白质的产生，以通过不同的生长阶段了解代谢优先级。在指数和静止生长阶段的每个实验培养物中都鉴定出了超过 2000 种蛋白质。在添加 C 标记亮氨酸的两个小时内，鲁杰氏菌显著地将新遇到的底物同化到新的蛋白质中。该数据集为理解普遍海洋细菌的分子生理学中的生长阶段差异提供了基本的基准。
