Partoandazanpoor Arjang, Sadeghi-Dehkordi Zainab, Ekradi Loghman, Khordadmehr Monireh, Rassouli Maryam, Sazmand Alireza
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Hamedan, 6517658978, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University, Sanandaj Branch, Sanandaj, Kordestan, Iran.
Acta Parasitol. 2020 Mar;65(1):187-192. doi: 10.2478/s11686-019-00147-4. Epub 2019 Dec 3.
Infection with the protozoan parasite Toxoplasma gondii is widely prevalent in animals and humans. In goats and sheep this infection has a high economic impact as it causes abortions and lamb losses. Although there are several studies reporting seroprevalence in small ruminants in Iran, molecular-based information is scarce.
This study aimed to screen caprine and ovine aborted fetuses for T. gondii infection by PCR and histopathology.
Brains of 121 aborted fetuses (10 caprine and 111 ovine) were collected from different parts of the Kordestan province, bordering with Iraq. Gestational age and the general status of each fetus such as freshness, autolysis, mummification and presence of macroscopic lesions in the fetus and foetal membranes was recorded. Individual brain tissues of fetuses were subjected to nested-PCR targeting the B1 gene, and histopathological sections prepared from brains were examined microscopically.
PCR results revealed T. gondii-associated abortion in one caprine and nine ovine fetuses (8.3%). Microscopically, pathological lesions included non-purulent meningitis associated with gliosis, focal necrosis, and occasionally tissue cyst.
This paper reports for the first time T. gondii-associated abortion in goats in Iran. As organs from aborted fetuses do not necessarily show lesions, molecular confirmation is the unique diagnostic method and should be used in situations of an abortion epidemic.
原生动物寄生虫刚地弓形虫感染在动物和人类中广泛流行。在山羊和绵羊中，这种感染具有很高的经济影响，因为它会导致流产和羔羊死亡。尽管有几项研究报告了伊朗小型反刍动物的血清阳性率，但基于分子的信息却很少。
本研究旨在通过聚合酶链反应（PCR）和组织病理学方法筛查山羊和绵羊流产胎儿是否感染刚地弓形虫。
从与伊拉克接壤的库尔德斯坦省不同地区收集了121例流产胎儿（10例山羊胎儿和111例绵羊胎儿）的大脑。记录每个胎儿的妊娠年龄以及一般状况，如新鲜度、自溶、木乃伊化以及胎儿和胎膜中是否存在宏观病变。对胎儿的单个脑组织进行靶向B1基因的巢式PCR检测，并对大脑制备的组织病理学切片进行显微镜检查。
PCR结果显示，1例山羊胎儿和9例绵羊胎儿（8.3%）存在与刚地弓形虫相关的流产。显微镜下，病理病变包括与胶质增生、局灶性坏死相关的非化脓性脑膜炎，偶尔还有组织囊肿。
本文首次报道了伊朗山羊中与刚地弓形虫相关的流产。由于流产胎儿的器官不一定会出现病变，分子确诊是唯一的诊断方法，应在流产流行的情况下使用。
