Neumann I, Schwarzberg H, Landgraf R
Department of Cell Biology and Regulation, Karl Marx University, Leipzig, G.D.R.
Brain Res. 1988 Oct 11;462(1):181-4. doi: 10.1016/0006-8993(88)90603-8.
The release of vasopressin (AVP) and oxytocin (OXT) within the septum was studied with the push-pull perfusion technique in 6 conscious, freely behaving male rats. Push-pull perfusion was performed via a chronically implanted cannula and samples collected for 3 consecutive 30-min periods. Stimulating electrodes were implanted in both the left and right paraventricular nuclei 4 days before the experiment. Bilateral electrical stimulation (10-s trains every 4 min) of the paraventricular nuclei during the second 30-min period resulted in a significant increase in the release of both AVP and OXT (128% and 159% of control values respectively); release returned to the pre-stimulation value during the final 30-min collection.
采用推挽式灌流技术，对6只清醒、自由活动的雄性大鼠中隔内血管加压素（AVP）和催产素（OXT）的释放情况进行了研究。通过长期植入的套管进行推挽式灌流，并连续3个30分钟时段收集样本。在实验前4天，将刺激电极植入左、右室旁核。在第二个30分钟时段对室旁核进行双侧电刺激（每4分钟10秒串刺激），导致AVP和OXT的释放均显著增加（分别为对照值的128%和159%）；在最后一个30分钟收集时段，释放量恢复到刺激前水平。
