光学相干断层扫描用于鸟类心脏发育过程中心内膜向间充质转变的成像。
Optical coherence tomography for imaging of endocardial to mesenchymal transition during avian heart development.
作者信息
Courchaine Katherine, Rugonyi Sandra
机构信息
Department of Biomedical Engineering, Oregon Health & Science University, 3303 SW Bond Ave. Mail/Code:CH13B, Portland, OR 97239, USA.
出版信息
Biomed Opt Express. 2019 Nov 1;10(11):5989-5995. doi: 10.1364/BOE.10.005989.
Abstract
The endocardial to mesenchymal transition (EndMT) that occurs in endocardial cushions during heart development is critical for proper heart septation and formation of the heart's valves. In EndMT, cells delaminate from the endocardium and migrate into the previously acellular endocardial cushions. Optical coherence tomography (OCT) imaging uses the optical properties of tissues for contrast, and during early development OCT can differentiate cellular versus acellular tissues. Here we show that OCT can be used to non-invasively track EndMT progression in the outflow tract cushions of chicken embryos. This enables studies to elucidate factors leading to cardiac malformations.
摘要
心脏发育过程中的心内膜垫发生的心内膜到间充质转变（EndMT）对于心脏正确的分隔和心脏瓣膜的形成至关重要。在EndMT过程中，细胞从心内膜脱离并迁移到先前无细胞的心内膜垫中。光学相干断层扫描（OCT）成像利用组织的光学特性进行对比，在早期发育过程中，OCT可以区分细胞组织和无细胞组织。在这里，我们表明OCT可用于无创追踪鸡胚流出道垫中的EndMT进程。这使得研究能够阐明导致心脏畸形的因素。
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