Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Food Funct. 2020 Jan 29;11(1):328-338. doi: 10.1039/c9fo01346f.
Skeletal muscle regeneration is a complicated process, requiring the proliferation, migration and differentiation of myoblasts whose processes are highly regulated by the extracellular matrix (ECM) surrounding the muscle tissues in vivo. However, the effects of respective ECM components on the regulation of myoblast behaviors are unknown. In this study, we report on the effect of collagen I, a major ECM component in muscle tissue and a popular food supplement, on mouse C2C12 myoblast proliferation, migration and differentiation as well as the underlying mechanisms. Collagen I (col 1) enhances the migration and myogenic differentiation of C2C12 cells, but has no effect on cell proliferation. Col I significantly promotes the production and release of interleukin-6 via nuclear translocation of nuclear factor κB (NF-κB) p65. The release of IL-6 plays a critical role in the col I-enhanced migration and differentiation of C2C12 cells. Furthermore, col I increases phosphorylation of focal adhesion kinase (FAK) that is involved in the nuclear translocation of NF-κB p65. Collectively, col I enhances the migration and differentiation of C2C12 cells through IL-6 release induced by FAK/NF-κB p65 activation.
骨骼肌再生是一个复杂的过程，需要肌母细胞的增殖、迁移和分化，其过程受到体内肌肉组织周围细胞外基质 (ECM) 的高度调节。然而，各自 ECM 成分对肌母细胞行为的调节作用尚不清楚。在这项研究中，我们报告了胶原蛋白 I（肌肉组织中的主要 ECM 成分和一种流行的食品补充剂）对小鼠 C2C12 肌母细胞增殖、迁移和分化的影响及其潜在机制。胶原蛋白 I（col 1）增强了 C2C12 细胞的迁移和成肌分化，但对细胞增殖没有影响。Col I 通过核因子 κB（NF-κB）p65 的核易位显著促进白细胞介素-6（IL-6）的产生和释放。IL-6 的释放在 col I 增强的 C2C12 细胞迁移和分化中起着关键作用。此外，col I 增加了黏着斑激酶（FAK）的磷酸化，这涉及 NF-κB p65 的核易位。总的来说，col I 通过 FAK/NF-κB p65 激活诱导的 IL-6 释放来增强 C2C12 细胞的迁移和分化。
