环状 RNA circ-SMAD7 通过上调 PCNA 促进神经胶质瘤细胞的增殖和转移。
Circular RNA circ-SMAD7 promoted glioma cell proliferation and metastasis by upregulating PCNA.
机构信息
Department of Neurosurgery, The Ninth People's Hospital of Suzhou, Suzhou, China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Nov;23(22):10035-10041. doi: 10.26355/eurrev_201911_19570.
OBJECTIVE
Recent studies have discovered a class of circular RNAs (circRNAs), which are dysregulated in various tumors and participate in the regulation of tumor progression. In our research, we aim to research the function of circ-SMAD7 in the progression of glioma.
PATIENTS AND METHODS
Circ-SMAD7 expression was detected by quantitative Real Time Polymerase Chain Reaction (qRT-PCR) in glioma tissue patients. Pearson's Chi-square test was used to determine the association of circ-SMAD7 expression with several clinicopathological factors. Besides, cell proliferation assay, cell cycle assay, transwell assay, and Matrigel assay were conducted to detect the function of circ-SMAD7 in glioma. In addition, the interaction between circ-SMAD7 and proliferating cell nuclear antigen (PCNA) in glioma was studied by performing qRT-PCR and Western blot assay.
RESULTS
Circ-SMAD7 expression was observed in glioma tissues when compared with adjacent samples. The expression of circ-SMAD7 was associated with patients' WHO stage and KPS score. Cell proliferation was inhibited and cell cycle was regulated after circ-SMAD7 was downregulated in glioma cells. Besides, cell migration and invasion were inhibited after circ-SMAD7 was downregulated in glioma cells. In addition, the mRNA and protein expression of PCNA was repressed after circ-SMAD7 was knocked down in glioma cells. Furthermore, PCNA expression level positively correlated to circ-SMAD7 expression level in glioma samples.
CONCLUSIONS
Our study suggests that circ-SMAD7 promotes proliferation and metastasis of glioma via upregulating PCNA. Circ-SMAD7/ PCNA might be a novel therapeutic strategy in glioma.
目的
最近的研究发现了一类环状 RNA（circRNAs），它们在各种肿瘤中失调，并参与肿瘤进展的调节。在我们的研究中，我们旨在研究 circ-SMAD7 在神经胶质瘤进展中的作用。
患者和方法
通过定量实时聚合酶链反应（qRT-PCR）检测神经胶质瘤组织患者中 circ-SMAD7 的表达。Pearson's Chi-square 检验用于确定 circ-SMAD7 表达与几种临床病理因素的关联。此外，进行细胞增殖测定、细胞周期测定、transwell 测定和 Matrigel 测定，以检测 circ-SMAD7 在神经胶质瘤中的功能。此外，通过 qRT-PCR 和 Western blot 测定研究 circ-SMAD7 和增殖细胞核抗原（PCNA）在神经胶质瘤中的相互作用。
结果
与相邻样本相比，在神经胶质瘤组织中观察到 circ-SMAD7 的表达。circ-SMAD7 的表达与患者的 WHO 分期和 KPS 评分有关。circ-SMAD7 在神经胶质瘤细胞中下调后，细胞增殖受到抑制，细胞周期得到调节。此外，circ-SMAD7 在神经胶质瘤细胞中下调后，细胞迁移和侵袭受到抑制。此外，circ-SMAD7 在神经胶质瘤细胞中敲低后，PCNA 的 mRNA 和蛋白表达受到抑制。此外，circ-SMAD7 在神经胶质瘤样本中的表达水平与 PCNA 的表达水平呈正相关。
结论
我们的研究表明，circ-SMAD7 通过上调 PCNA 促进神经胶质瘤的增殖和转移。circ-SMAD7/PCNA 可能是神经胶质瘤的一种新的治疗策略。
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