环状RNA hsa_circ_0011946通过上调增殖细胞核抗原促进口腔鳞状细胞癌的细胞生长、迁移和侵袭。
Circular RNA hsa_circ_0011946 promotes cell growth, migration, and invasion of oral squamous cell carcinoma by upregulating PCNA.
作者信息
Meng Y, Zhao E-Y, Zhou Y, Qiang D-X, Wang S, Shi L, Jiang L-Y, Bi L-J
机构信息
Department of Stomatology, the Fourth Affiliated Hospital, Harbin Medical University, Harbin, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Feb;24(3):1226-1232. doi: 10.26355/eurrev_202002_20175.
OBJECTIVE
The importance of circular RNAs in malignant tumors has been well concerned nowadays. Oral squamous cell carcinoma (OSCC) is diagnosed prevalently in the world. Our study aims to uncover the potential functions of hsa_circ_0011946 in OSCC development.
PATIENTS AND METHODS
Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was performed to determine the level of hsa_circ_0011946 in OSCC tissues and cell lines. Hsa_circ_0011946 was knocked down in OSCC cells. Biological functions of hsa_circ_0011946 in OSCC were identified by performing cell proliferation assay, colony formation assay, wound healing assay, and transwell assay. The underlying mechanism of hsa_circ_0011946 in regulating OSCC progression was explored by RT-qPCR and Western blot assay.
RESULTS
Hsa_circ_0011946 was highly expressed in OSCC tissues compared with adjacent samples. It was also upregulated in OSCC cell lines. The knockdown of hsa_circ_0011946 inhibited cell growth, migration, and invasion in OSCC. The expression of PCNA was reduced via knockdown of hsa_circ_0011946. Furthermore, the expression of PCNA in tumor tissues was positively correlated to the expression of hsa_circ_0011946.
CONCLUSIONS
Hsa_circ_0011946 could promote cell growth, migration, and invasion of OSCC by upregulating PCNA, which may offer a new therapeutic intervention for OSCC patients.
目的
环状RNA在恶性肿瘤中的重要性如今已备受关注。口腔鳞状细胞癌（OSCC）在全球范围内普遍被诊断出来。我们的研究旨在揭示hsa_circ_0011946在OSCC发展中的潜在功能。
患者和方法
采用实时定量聚合酶链反应（RT-qPCR）来测定OSCC组织和细胞系中hsa_circ_0011946的水平。在OSCC细胞中敲低hsa_circ_0011946。通过进行细胞增殖试验、集落形成试验、伤口愈合试验和Transwell试验来鉴定hsa_circ_0011946在OSCC中的生物学功能。通过RT-qPCR和蛋白质免疫印迹试验探索hsa_circ_0011946调节OSCC进展的潜在机制。
结果
与相邻样本相比，hsa_circ_0011946在OSCC组织中高表达。它在OSCC细胞系中也上调。敲低hsa_circ_0011946可抑制OSCC细胞的生长、迁移和侵袭。通过敲低hsa_circ_0011946，增殖细胞核抗原（PCNA）的表达降低。此外，肿瘤组织中PCNA的表达与hsa_circ_0011946的表达呈正相关。
结论
hsa_circ_0011946可通过上调PCNA促进OSCC细胞的生长、迁移和侵袭，这可能为OSCC患者提供一种新的治疗干预措施。
Zotero 插件