Slattery Martha L, Herrick Jennifer S, Mullany Lila E, Stevens John R, Wolff Roger K
Department of Internal Medicine, The University of Utah, Salt Lake City.
Department of Mathematics and Statistics, Utah State University, Logan, UT, USA.
Pharmgenomics Pers Med. 2016 Dec 20;10:1-16. doi: 10.2147/PGPM.S117796. eCollection 2017.
MicroRNAs (miRNAs) are small non-protein-coding RNA molecules that regulate gene expression. Diet and lifestyle factors have been hypothesized to be involved in the regulation of miRNA expression. In this study it was hypothesized that diet and lifestyle factors are associated with miRNA expression. Data from 1,447 cases of colorectal cancer to evaluate 34 diet and lifestyle variables using miRNA expression in normal colorectal mucosa as well as for differential expression between paired carcinoma and normal tissue were used. miRNA data were obtained using an Agilent platform. Multiple comparisons were adjusted for using the false discovery rate -value. There were 250 miRNAs differentially expressed between carcinoma and normal colonic tissue by level of carbohydrate intake and 198 miRNAs differentially expressed by the level of sucrose intake. Of these miRNAs, 166 miRNAs were differentially expressed for both carbohydrate intake and sucrose intake. Ninety-nine miRNAs were differentially expressed by the level of whole grain intake in normal colonic mucosa. Level of oxidative balance score was associated with 137 differentially expressed miRNAs between carcinoma and paired normal rectal mucosa. Additionally, 135 miRNAs were differentially expressed in colon tissue based on recent NSAID use. Other dietary factors, body mass index, waist and hip circumference, and long-term physical activity levels did not alter miRNA expression after adjustment for multiple comparisons. These results suggest that diet and lifestyle factors regulate miRNA level. They provide additional support for the influence of carbohydrate, sucrose, whole grains, NSAIDs, and oxidative balance score on colorectal cancer risk.
微小RNA（miRNA）是一类调节基因表达的非蛋白质编码小分子RNA。据推测，饮食和生活方式因素参与了miRNA表达的调控。在本研究中，我们假设饮食和生活方式因素与miRNA表达相关。研究使用了1447例结直肠癌患者的数据，通过正常结直肠黏膜中的miRNA表达以及配对癌组织与正常组织之间的差异表达来评估34种饮食和生活方式变量。miRNA数据通过安捷伦平台获得。采用错误发现率值对多重比较进行校正。根据碳水化合物摄入量水平，癌组织与正常结肠组织之间有250种miRNA差异表达；根据蔗糖摄入量水平，有198种miRNA差异表达。在这些miRNA中，有166种miRNA在碳水化合物摄入量和蔗糖摄入量水平上均有差异表达。在正常结肠黏膜中，根据全谷物摄入量水平有99种miRNA差异表达。氧化平衡评分水平与癌组织和配对正常直肠黏膜之间137种差异表达的miRNA相关。此外，根据近期非甾体抗炎药（NSAID）的使用情况，结肠组织中有135种miRNA差异表达。在对多重比较进行校正后，其他饮食因素、体重指数、腰围和臀围以及长期身体活动水平均未改变miRNA表达。这些结果表明，饮食和生活方式因素调节miRNA水平。它们为碳水化合物、蔗糖、全谷物、NSAIDs和氧化平衡评分对结直肠癌风险的影响提供了额外的支持。
