温肾汤通过抑制2型固有淋巴细胞（ILC2）激活，抑制白细胞介素-33（IL-33）诱导的哮喘小鼠模型中的炎症反应。
Wenshen decoction suppresses inflammation in IL-33-induced asthma murine model via inhibiting ILC2 activation.
作者信息
Huang Weiling, Song Ying, Wang Lixin
机构信息
Department of Respiration, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China.
Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
出版信息
Ann Transl Med. 2019 Oct;7(20):570. doi: 10.21037/atm.2019.09.34.
BACKGROUND
Wenshen decoction, consisting of Epimedium brevicornu Maxim, Morinda officinalis How and Cnidium monnieri (L.) Cusson, has favorable efficacy in the treatment of asthma in China.
METHODS
The present study investigated the potential immunomodulatory mechanism underlying the therapeutic effects of Wenshen decoction on mouse asthma.
RESULTS
Oral Wenshen decoction could ameliorate the production of IL-4, IL-5, IL-13 in the bronchoalveolar lavage fluid (BALF), reduce serum IgE, and improve the airway hyperresponsiveness (AHR) and airway inflammation in the BALB/c mice after intranasal treatment with recombinant IL-33. Moreover, Wenshen decoction reduced ILC2 and RORα mRNA expression, decreased the mRNA expression of ICOS and ST2 in the lung, but significantly increased the production of IFN-γ.
CONCLUSIONS
Our study indicates that Wenshen decoction may inhibit the activation of ILC2 through the IL-33/ST2/ICOS pathway to further suppress airway inflammation and AHR in the asthmatic mice, and the increased IFN-γ might be related to the effects of Wenshen decoction on ILC2.
背景
由淫羊藿、巴戟天和蛇床子组成的温肾汤在中国治疗哮喘方面具有良好疗效。
方法
本研究探讨温肾汤对小鼠哮喘治疗作用的潜在免疫调节机制。
结果
口服温肾汤可改善支气管肺泡灌洗液（BALF）中白细胞介素-4（IL-4）、白细胞介素-5（IL-5）、白细胞介素-13的产生，降低血清免疫球蛋白E（IgE），并改善重组白细胞介素-33鼻内给药后BALB/c小鼠的气道高反应性（AHR）和气道炎症。此外，温肾汤降低了2型固有淋巴细胞（ILC2）和维甲酸相关孤儿受体α（RORα）的信使核糖核酸（mRNA）表达，降低了肺中诱导性共刺激分子（ICOS）和白细胞介素-33受体（ST2）的mRNA表达，但显著增加了干扰素-γ（IFN-γ）的产生。
结论
我们的研究表明，温肾汤可能通过白细胞介素-33/ST2/ICOS途径抑制ILC2的激活，从而进一步抑制哮喘小鼠的气道炎症和AHR，而IFN-γ的增加可能与温肾汤对ILC2的作用有关。
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