Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Pesquisa em Farmacologia Bioquímica (LaFarBio), Grupo de Pesquisa em Neurobiotecnologia (GPN), Universidade Federal de Pelotas, CEP 96010-900 Pelotas, RS, Brazil.
Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Pesquisa em Farmacologia Bioquímica (LaFarBio), Grupo de Pesquisa em Neurobiotecnologia (GPN), Universidade Federal de Pelotas, CEP 96010-900 Pelotas, RS, Brazil.
Exp Gerontol. 2020 Feb;130:110804. doi: 10.1016/j.exger.2019.110804. Epub 2019 Dec 4.
This study investigated whether subacute treatment with 7-chloro-4-(phenylselanyl) quinoline (4-PSQ) presented antioxidant action in oxidative stress associated with aging in different rat tissues. We also investigated whether plasma selenium levels were altered by aging, as well as the contribution of 4-PSQ administration to these levels. Aged Wistar male rats (23month old) were intragastrically treated with 4-PSQ (5mg/kg) for seven days. On the 14th day of the experimental protocol, plasma was collected to determine selenium levels and biochemical markers of renal and hepatic damage. Furthermore, liver, kidney, spleen and cerebral cortex were removed to determine thiobarbituric acid reactive species (TBARS), non-protein thiols (NPSH), glutathione S-transferase (GST), catalase (CAT) and δ-aminolevulinate dehydratase (ALA-D). Our results showed that one or more parameters changed markedly in the liver, kidney, spleen and cerebral cortex of aged rats. Moreover, biochemical markers of renal and hepatic damage and selenium levels are changed in the plasma of aged rats. Treatment with 4-PSQ repaired redox homeostasis in tissues of aged rats, as well as plasma biochemical markers of renal and hepatic damage and selenium levels. In conclusion, 4-PSQ presented an antioxidant effect in tissues of aged rats, restoring selenium levels, and contributing to the restoration of the damage caused by aging. Thus, 4-PSQ could be a potential candidate for the management of age-related oxidative damage, acting as an anti-aging drug.
本研究旨在探讨亚急性给予 7-氯-4-(苯硒基)喹啉（4-PSQ）是否对衰老相关氧化应激引起的不同大鼠组织中的抗氧化作用。我们还研究了衰老是否会改变血浆硒水平，以及 4-PSQ 给药对这些水平的影响。雄性 Wistar 大鼠（23 月龄）经口给予 4-PSQ（5mg/kg）7 天。在实验方案的第 14 天，采集血浆以测定硒水平和肾、肝损伤的生化标志物。此外，取出肝脏、肾脏、脾脏和大脑皮质，以测定丙二醛（TBARS）、非蛋白巯基（NPSH）、谷胱甘肽 S-转移酶（GST）、过氧化氢酶（CAT）和 δ-氨基酮戊酸脱水酶（ALA-D）。我们的结果表明，衰老大鼠的肝脏、肾脏、脾脏和大脑皮质中的一个或多个参数发生了明显变化。此外，衰老大鼠血浆中的肾、肝损伤生化标志物和硒水平也发生了变化。4-PSQ 治疗修复了衰老大鼠组织中的氧化还原平衡，以及血浆肾、肝损伤生化标志物和硒水平。综上所述，4-PSQ 对衰老大鼠组织具有抗氧化作用，恢复了硒水平，并有助于修复衰老引起的损伤。因此，4-PSQ 可能是管理与年龄相关的氧化损伤的潜在候选药物，可作为一种抗衰老药物。
