Mamishi Setareh, Mohammadian Maryam, Pourakbari Babak, Hosseinpour Sadeghi Reihaneh, Haghi Ashtiani Mohammad Taghi, Abdosalehi Mohammad Reza, Rahmani Maryam, Mahmoudi Shima
Department of Infectious Diseases, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Pediatric Infectious Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Infect Drug Resist. 2019 Nov 27;12:3719-3726. doi: 10.2147/IDR.S220522. eCollection 2019.
A serious problem affecting human society is the development of bacterial resistance. The purpose of the current study was to evaluate the antibiotic resistance of Gram-positive bacteria (GPB) and genotyping of common GPB causing hospital-acquired infections (HAIs) in patients who were referred to Children's Medical Center during a 6-month period by random amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (ERIC-PCR).
During the 6-month period, antimicrobial resistance profiles of GPB isolates recovered from patients in Children's Medical Center were determined using the Kirby-Bauer disk diffusion and MIC. Typing of common GPB was performed and the results were analyzed by gel compare software.
In this cross-sectional study, 6524 cultures were performed and 138 Ggram-positive bacteria were isolated (2%). strains showed the highest antibiotic penicillin resistance (96.3%). Twenty-six per cent of the strains were methicillin-resistant (MRSA) and no resistance was found against vancomycin. All isolates of were resistant to ciprofloxacin (100%). The resistance to vancomycin was very high (67%) and no resistance was observed to linezolid. The results of genotyping analysis of strains showed the presence of two clones with a genetic relationship of over 80%. All of the strains were in one cluster and half of the strains were in a cluster with a genetic predilection of over 80%.
This study indicated frequent occurrence of antimicrobial resistance, especially in spp. isolates. Rapid spreads of MRSA and VREF from a clonal origin require implementing careful isolation and infection control measures.
影响人类社会的一个严重问题是细菌耐药性的发展。本研究的目的是通过随机扩增多态性DNA（RAPD）和肠杆菌重复基因间共识序列聚合酶链反应（ERIC-PCR），评估革兰氏阳性菌（GPB）的抗生素耐药性以及在6个月期间转诊至儿童医学中心的患者中引起医院获得性感染（HAIs）的常见GPB的基因分型。
在这6个月期间，使用 Kirby-Bauer 纸片扩散法和 MIC 测定从儿童医学中心患者中分离出的 GPB 分离株的抗菌药物耐药谱。对常见的 GPB 进行分型，并通过凝胶比较软件分析结果。
在这项横断面研究中，共进行了6524次培养，分离出138株革兰氏阳性菌（2%）。菌株显示出最高的青霉素耐药率（96.3%）。26%的菌株对甲氧西林耐药（MRSA），未发现对万古霉素耐药。所有分离株均对环丙沙星耐药（100%）。对万古霉素的耐药性非常高（67%），未观察到对利奈唑胺的耐药性。菌株的基因分型分析结果显示存在两个克隆，其遗传关系超过80%。所有菌株都在一个簇中，一半的菌株在一个遗传倾向超过80%的簇中。
本研究表明抗菌药物耐药性频繁发生，尤其是在菌株分离物中。MRSA 和 VREF 从克隆起源的快速传播需要实施仔细的隔离和感染控制措施。
