Mamishi Setareh, Akhlaghi Arezu, Pourakbari Babak, Modaresi Mohammadreza, Haghi Ashtiani Mohammad Taghi, Hosseinpour Sadeghi Reihaneh, Shirzadi Rohola, Shalchi Zohreh, Mahmoudi Shima
Pediatric Infectious Diseases Research Center, Children's Medical Center Hospital, School of Medicine, Tehran University of Medical Sciences, No.62, Gharib St., Keshavarz Blvd., Tehran, Iran.
Department of Infectious Diseases, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Wien Med Wochenschr. 2023 May;173(7-8):182-187. doi: 10.1007/s10354-022-00970-x. Epub 2022 Sep 27.
Chronic bacterial infections of the airways are present in most patients with cystic fibrosis (CF). Although most pathogens are acquired from the environment, there is great evidence of patient-to-patient transmission. Therefore, evaluating the genetic variation of strains isolated from CF patients is recommended for the purpose of examining hospital infection.
The aim of this study was to determine the antibiotic susceptibility pattern and genotyping of Staphylococcus aureus and Pseudomonas aeruginosa strains isolated from sputum samples of children with CF referred to a single pediatric CF center in Tehran, Iran.
In this cross-sectional study, the antimicrobial susceptibility profiles of strains isolated from patients with CF during 1 year were determined. Pseudomonas aeruginosa and S. aureus isolates were genotyped using the random amplified polymorphic DNA polymerase chain reaction method and were analyzed using GelCompar II software.
Of 534 patients with CF, 384 had negative sputum cultures (72%), and 94 strains of P. aeruginosa (18%) and 53 strains of S. aureus (10%) were isolated. The mean age of the patients was 8.22 ± 5.7 years (range, 2 months to 18 years). The P. aeruginosa strains showed high sensitivity to ceftazidime (96%), piperacillin/tazobactam (96%), and imipenem (94%). All strains of S. aureus were susceptible to vancomycin, and 13% of the strains were methicillin-resistant S. aureus. High resistance to penicillin (92%) and erythromycin (88.5%) were reported. The results of P. aeruginosa genotyping revealed that there were six major clusters in this hospital. Also, based on the analysis of genotyping results, S. aureus strains were obtained from five clusters, most of which were located in cluster B1 (34 isolates, 64%).
The results of this study show the possibility of strains being transferred from one part of the hospital to another (especially from the respiratory ward to other areas). Hence, high attention should be paid to the basic methods of preventing infection.
大多数囊性纤维化（CF）患者存在气道慢性细菌感染。虽然大多数病原体是从环境中获得的，但有充分证据表明存在患者间传播。因此，为检查医院感染情况，建议评估从CF患者中分离出的菌株的基因变异。
本研究的目的是确定从伊朗德黑兰一家儿科CF中心转诊的CF患儿痰液样本中分离出的金黄色葡萄球菌和铜绿假单胞菌菌株的抗生素敏感性模式和基因分型。
在这项横断面研究中，确定了1年内从CF患者中分离出的菌株的抗菌药物敏感性概况。使用随机扩增多态性DNA聚合酶链反应方法对铜绿假单胞菌和金黄色葡萄球菌分离株进行基因分型，并使用GelCompar II软件进行分析。
在534例CF患者中，384例痰培养阴性（72%），分离出94株铜绿假单胞菌（18%）和53株金黄色葡萄球菌（10%）。患者的平均年龄为8.22±5.7岁（范围为2个月至18岁）。铜绿假单胞菌菌株对头孢他啶（96%）、哌拉西林/他唑巴坦（96%）和亚胺培南（94%）表现出高敏感性。所有金黄色葡萄球菌菌株对万古霉素敏感，13%的菌株为耐甲氧西林金黄色葡萄球菌。据报道对青霉素（92%）和红霉素（88.5%）的耐药性较高。铜绿假单胞菌基因分型结果显示，该医院存在六个主要聚类。此外，根据基因分型结果分析，金黄色葡萄球菌菌株来自五个聚类，其中大部分位于B1聚类（34株，64%）。
本研究结果表明菌株有可能从医院的一个区域转移到另一个区域（特别是从呼吸病房转移到其他区域）。因此，应高度重视预防感染的基本方法。
