Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland.
Interdisciplinary Centre for Modern Technologies, Nicolaus Copernicus University, Toruń, Poland.
Crit Rev Anal Chem. 2021;51(2):150-173. doi: 10.1080/10408347.2019.1699013. Epub 2019 Dec 10.
A chromatographic column is the fundamental element required for gas-chromatographic analysis. The separation of components coming from complex mixtures, prior to their detection was leading to a prominent revolution in different areas of science. Moreover, current advances in gas chromatographic (GC) columns technology and development have been providing almost unlimited possibilities for analysis employing diverse matrices. We aim through this review article to describe the evolution of chromatographic columns, by pointing the most important stages, as well as the new trends and future perspectives predicted for the new generation of GC columns. Furthermore, it was in our scope to present the main fundamentals regarding the theoretical relationships that describe the chromatographic separation, to introduce concepts related to columns selection in accordance with the required application as well as to discuss the available evaluation parameters for columns efficiency. Consequently, the early stages of first columns preparation up to the development of GC capillary columns used nowadays, together with examples of their applications are also reported and described in detail.
色谱柱是气相色谱分析所必需的基本要素。对来自复杂混合物的成分进行分离,在它们被检测之前,这在不同的科学领域引发了一场显著的革命。此外,气相色谱(GC)柱技术和发展的当前进展为采用不同基质的分析提供了几乎无限的可能性。我们的目的是通过这篇综述文章来描述色谱柱的发展,指出最重要的阶段,以及预测新一代 GC 柱的新趋势和未来展望。此外,我们还介绍了有关描述色谱分离的理论关系的主要基础,引入了与根据所需应用选择柱子相关的概念,并讨论了柱子效率的可用评估参数。因此,详细报告并描述了从第一根柱子的早期制备到现今使用的 GC 毛细管柱子的发展,以及它们的应用实例。