Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China.
Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China.
Bioelectrochemistry. 2020 Apr;132:107434. doi: 10.1016/j.bioelechem.2019.107434. Epub 2019 Nov 30.
In this study, a novel signal-amplified immunosensor was designed by using a microwave-assisted self-assembly method to synthesize ZnFeO-Ag/rGO nanocomposites. The conductivity of ZnFeO-rGO nanocomposites was significantly improved due to the effective inhibition of rGO accumulation by the insertion of ZnFeO and Ag nanoparticles (NPs) into graphene sheets. Excellent sensitivity and reproducibility were achieved through the microwave-assisted preparation of ZnFeO-Ag/rGO nanocomposites as a substrate, with the Ag NPs enhancing the signal because of the effective conductive matrix. The layer assembly process of the immunosensor was verified by cyclic voltammetry and electrochemical impedance spectroscopy. Under optimal conditions, the fabricated immunosensor showed good linearity over a wide concentration range from 1 pg mL to 200 ng mL with a low detection limit of 0.98 pg mL, and exhibited excellent specificity, good stability, and reproducibility. These qualities can contribute to the successful application of a label-free immunosensor in the detection of AFP in human serum.
在这项研究中，我们通过微波辅助自组装方法设计了一种新型信号放大免疫传感器，用于合成 ZnFeO-Ag/rGO 纳米复合材料。由于 ZnFeO 和 Ag 纳米颗粒 (NPs) 插入到石墨烯片层中有效抑制了 rGO 的堆积，ZnFeO-rGO 纳米复合材料的导电性得到了显著提高。通过微波辅助制备 ZnFeO-Ag/rGO 纳米复合材料作为基底，Ag NPs 增强了信号，实现了优异的灵敏度和重现性。通过循环伏安法和电化学阻抗谱验证了免疫传感器的层组装过程。在最佳条件下，制备的免疫传感器在 1 pg mL 至 200 ng mL 的宽浓度范围内表现出良好的线性关系，检测限低至 0.98 pg mL，并且表现出优异的特异性、良好的稳定性和重现性。这些特性有助于实现无标记免疫传感器在人血清 AFP 检测中的成功应用。
