Sayed Khulood Muhammad, Mostafa Engy Mohammed, Mounir Amr, Abd Elhaliem Nesreen G, Alsmman Alahmady Hamad
The Department of Ophthalmology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt.
The Department of Histology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt.
Clin Ophthalmol. 2019 Dec 5;13:2421-2426. doi: 10.2147/OPTH.S229335. eCollection 2019.
To analyze ultrastructural changes of rabbits' eyelash follicles treated with bimatoprost eye drops to increase our knowledge of how this drug works.
The study included 15 clinically healthy male rabbits. All rabbits were treated with bimatoprost 0.03% daily for 4 weeks with one drop of the topical eye drops applied to the conjunctival fornix of the right eyes; left eyes were used as controls. Eyelash lengths were measured before and after treatment. The eyelid of each animal was dissected for light and electron microscopic analysis.
Both control and treated rabbit eyes matched regarding eyelash length before treatment (9.80±0.388mm vs 9.88±0.24mm) (P=0.108). There was a significant increase in eyelash length between control (9.75±0.33 mm) and treated rabbit eyes (11.60±0.46 mm) (P=0.369). Light and electron microscopy revealed, bimatoprost treated eyes had thick epidermis. The dermis contained two hairs growing out of the same hair follicle. Heavily keratinized Henle's layer, the cortical cells (Cx) have prominent nucleolus and cytoplasm is studded with melanin granules.
Bimatoprost-induced eyelash changes were not restricted to increased eyelash length, thickness, and pigmentation but also showed increased number of eyelashes within the same hair follicle which were stronger and could resist pulling from the skin without any evidence of inflammatory cells within the specimens. These changes occurred as early as 1 month of treatment, giving rise to thoughts about the possibility of using bimatoprost eye drops as a prophylaxis against madarosis associated with chemotherapy if it is started 1 month before chemotherapy and continued afterwards, making eyelashes stronger and resistant to falling out.
分析用比马前列素滴眼液治疗的兔睫毛毛囊的超微结构变化，以增进我们对该药物作用机制的了解。
该研究纳入了15只临床健康的雄性兔。所有兔每日用0.03%的比马前列素治疗4周，将一滴局部滴眼液滴入右眼结膜穹窿；左眼用作对照。在治疗前后测量睫毛长度。解剖每只动物的眼睑进行光镜和电镜分析。
治疗前，对照兔眼和治疗兔眼的睫毛长度匹配（9.80±0.388毫米对9.88±0.24毫米）（P = 0.108）。对照兔眼（9.75±0.33毫米）和治疗兔眼（11.60±0.46毫米）的睫毛长度有显著增加（P = 0.369）。光镜和电镜显示，用比马前列素治疗的兔眼表皮增厚。真皮中有两根毛发从同一个毛囊中长出。亨勒层重度角化，皮质细胞（Cx）有明显的核仁，细胞质中布满黑色素颗粒。
比马前列素引起的睫毛变化不仅限于睫毛长度、厚度和色素沉着增加，还表现为同一毛囊内睫毛数量增加，睫毛更强壮，能抵抗从皮肤上拔出，且标本内无任何炎症细胞迹象。这些变化早在治疗1个月时就出现，这引发了一种想法，即如果在化疗前1个月开始使用比马前列素滴眼液并在化疗后持续使用，有可能将其用作预防与化疗相关的睫毛脱落的药物，使睫毛更强壮且不易脱落。
