Colombe Laurent, Vindrios Armelle, Michelet Jean-François, Bernard Bruno Alain
Centre Charles Zviak, L'Oreal Recherche, 90 rue du Général Roguet, Clichy Cedex, France.
Exp Dermatol. 2007 Sep;16(9):762-9. doi: 10.1111/j.1600-0625.2007.00586.x.
Prostaglandins regulate a wide number of physiological functions. Recently PGF(2alpha) analogue such as latanoprost was shown to have a real impact on hair regrowth. The aim of this study was to investigate and describe the expression profile in human hair follicle of prostaglandin metabolism key enzymes, i.e. carbonyl reductase-1 (CBR1), microsomal prostaglandin E synthase-1 (mPGES-1) and microsomal prostaglandin E synthase-2 (mPGES-2), cytosolic prostaglandin E synthase (cPGES), the aldoketoreductase AKR1C1 and the prostaglandin F synthase AKR1C3. Quantitative RT-PCR on plucked hair follicles revealed some sex-related differences, mPGES-2 and AKR1C3 expression levels being higher in women. Cell and hair follicle compartment specificity was investigated using Western blot, PGE(2) and PGF(2alpha) ELISA assays and immunohistochemistry. Most of the hair cell types were endowed with prostaglandin metabolism machinery and were thus able to produce PGE(2) and/or PGF(2alpha). The epithelial part of the hair bulb was identified by immunohistology and EIA assays as the main source of prostaglandin synthesis and interconversion. All these observations support the concept that prostaglandins might be involved in hair growth and differentiation control.
前列腺素调节多种生理功能。最近，诸如拉坦前列素之类的前列腺素F2α类似物已被证明对毛发生长有实际影响。本研究的目的是调查和描述前列腺素代谢关键酶，即羰基还原酶-1（CBR1）、微粒体前列腺素E合酶-1（mPGES-1）、微粒体前列腺素E合酶-2（mPGES-2）、胞质前列腺素E合酶（cPGES）、醛酮还原酶AKR1C1和前列腺素F合酶AKR1C3在人毛囊中的表达谱。对拔下的毛囊进行定量逆转录聚合酶链反应揭示了一些性别相关差异，女性的mPGES-2和AKR1C3表达水平较高。使用蛋白质免疫印迹法、前列腺素E2和前列腺素F2α酶联免疫吸附测定法以及免疫组织化学研究了细胞和毛囊区室特异性。大多数毛发细胞类型都具备前列腺素代谢机制，因此能够产生前列腺素E2和/或前列腺素F2α。通过免疫组织学和酶免疫测定法确定毛球的上皮部分是前列腺素合成和相互转化的主要来源。所有这些观察结果都支持前列腺素可能参与毛发生长和分化控制这一概念。
