Department of Biomedical and Analytical Sciences, Fujita Health University School of Medical Sciences, 1-98, Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
Department of Medical Technology, Kagawa Prefectural University of Health Sciences, 281-1, Murechohara, Takamatsu, Kagawa, 761-0123, Japan.
Sci Rep. 2019 Dec 11;9(1):18856. doi: 10.1038/s41598-019-55076-z.
Non-alcoholic fatty liver disease (NAFLD) is closely associated with obesity, metabolic syndrome, and type II diabetes mellitus. Recently, circulating microRNAs (miRNAs) have been proposed as useful disease biomarkers. We examined whether circulating miRNAs, such as miR-20a, miR-27a, and miR-126, were useful biomarkers for NAFLD. We conducted a cross-sectional analysis of 527 subjects aged 39 years or older who had undergone a health examination in the Yakumo Study. Of the residents, 92 were diagnosed with NAFLD using a registered medical sonographer. Serum miR-20a, miR-27a and miR-126 levels were measured by quantitative real-time PCR. We then calculated the odds ratios for serum miRNA level changes according to the severity of NAFLD using normal liver status as the reference group. Serum levels of miR-20a and 27a, but not miR-126, were significantly lower in NAFLD subjects than normal subjects. Serum miR-20a and miR-27a levels were significantly lower in both male and female severe NAFLD subjects. Logistic regression analysis showed a significant relationship between low circulating miR-20a and 27a levels and severe NAFLD. Down-regulated circulating miR-20a and 27a levels were significantly associated with severe NAFLD in the general population. Circulating miR-20a and miR-27a may be useful biomarkers for severe NAFLD.
非酒精性脂肪性肝病（NAFLD）与肥胖、代谢综合征和 2 型糖尿病密切相关。最近，循环 microRNAs（miRNAs）被认为是有用的疾病生物标志物。我们研究了循环 miRNAs，如 miR-20a、miR-27a 和 miR-126 是否可作为 NAFLD 的有用生物标志物。我们对在屋久研究中接受健康检查的 527 名年龄在 39 岁或以上的居民进行了横断面分析。其中，92 名居民被注册的医学超声医师诊断为 NAFLD。通过定量实时 PCR 测量血清 miR-20a、miR-27a 和 miR-126 水平。然后，我们根据 NAFLD 的严重程度计算了血清 miRNA 水平变化的比值比，以正常肝脏状态为参考组。与正常组相比，NAFLD 组的血清 miR-20a 和 miR-27a 水平显著降低，但 miR-126 水平无显著差异。男性和女性严重 NAFLD 患者的血清 miR-20a 和 miR-27a 水平均显著降低。logistic 回归分析显示，低循环 miR-20a 和 miR-27a 水平与严重 NAFLD 之间存在显著关系。下调的循环 miR-20a 和 miR-27a 水平与一般人群中严重的 NAFLD 显著相关。循环 miR-20a 和 miR-27a 可能是严重 NAFLD 的有用生物标志物。
