The Key Laboratory, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang 314000, China.
Clinical Laboratory, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang 314000, China.
Biomed Res Int. 2019 Nov 15;2019:7196535. doi: 10.1155/2019/7196535. eCollection 2019.
Polydatin (PD), an active component of Chinese herbs, is reported to have many biological functions, such as cardioprotective actions, anti-inflammatory activities, and antitumor effects. In this study, we investigated the effects of PD on body weight control, glucose and lipid metabolic regulation, and anti-inflammation in a high-fat-diet- (HFD-) induced obese mice model. After treatment of PD (100 mg/kg/d for 4 weeks), HFD mice reduced body weight, retroperitoneal fat mass, and adipose cell sizes; significantly lowered serum total cholesterol triglyceride (TG) and low-density lipoprotein (LDL) levels; and increased high-density lipoprotein (HDL) levels compared with the HFD control mice. Further studies showed that PD downregulated the mRNA and protein expressions of peroxisome proliferator-activated receptor gamma (PPAR), a transcription factor involving in the regulation of adipocyte differentiation, in the retroperitoneal fat of HFD mice. Additionally, PD significantly upregulated the mRNA and protein expressions of leptin, an adipocyte-derived anorexic hormone that regulates food intake and energy expenditure, in the adipose tissues of HFD mice. Moreover, PD reduced the expression levels of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF-) in the retroperitoneal and epididymal tissues of HFD mice, suggesting that PD prevented adipose tissue inflammation. In conclusion, PD may serve as a pharmaceutic candidate for obesity-related lipid metabolism, anti-inflammation, and body weight loss.
虎杖苷（PD）是一种中药的活性成分，据报道具有许多生物学功能，如心脏保护作用、抗炎活性和抗肿瘤作用。在这项研究中，我们研究了 PD 对高脂肪饮食（HFD）诱导肥胖小鼠模型体重控制、葡萄糖和脂质代谢调节以及抗炎的影响。经过 PD（100mg/kg/d 治疗 4 周）处理后，HFD 小鼠体重、腹膜后脂肪量和脂肪细胞大小降低；血清总胆固醇甘油三酯（TG）和低密度脂蛋白（LDL）水平显著降低；高密度脂蛋白（HDL）水平升高与 HFD 对照组相比。进一步的研究表明，PD 下调了 HFD 小鼠腹膜后脂肪中参与调节脂肪细胞分化的过氧化物酶体增殖物激活受体γ（PPAR）的 mRNA 和蛋白表达。此外，PD 还显著上调了 HFD 小鼠脂肪组织中瘦素的 mRNA 和蛋白表达，瘦素是一种由脂肪细胞分泌的厌食激素，可调节食物摄入和能量消耗。此外，PD 降低了 HFD 小鼠腹膜后和附睾组织中单核细胞趋化蛋白-1（MCP-1）和肿瘤坏死因子-α（TNF-α）的表达水平，表明 PD 可预防脂肪组织炎症。总之，PD 可能是一种与肥胖相关的脂质代谢、抗炎和体重减轻的药物候选物。
