Department of Pharmacology, College of Pharmacy, Harbin Medical University, Harbin, China.
State-Province Key Laboratory of Biopharmaceutical Engineering, Harbin, China.
Curr Pharm Des. 2019;25(46):4919-4927. doi: 10.2174/1381612825666191212105145.
Anthocyanins are a type of flavonoids that are natural water soluble glycosidic pigments with efficacious anti-cancer effects, which have good biological activity against many cancers including colorectal cancer (CRC). However, the exact molecular mechanism used by anthocyanins against cancer is unclear; it is also unclear what a reasonable dosage might be for their use against colorectal cancer.
Western blotting, immunohistochemistry, MTT assay, xenograft model, and hematoxylin-eosin (HE) staining were used to perform the experiments.
Compared with the control group, anthocyanins could significantly inhibit the cell viability and proliferation and promote the apoptosis of human colon cancer HT29 cells. Furthermore, anthocyanins reduced tumor weight and volume in a colon tumor mouse model and downregulated the expression of PI3K protein, inhibited AKT expression and phosphorylation, decreased the Bcl-2 and Bax ratio and reduced survivin protein expression in the tumor tissue.
Anthocyanins promoted apoptosis of CRC cells and inhibited colon cancer growth of xenografted tumors. Mechanistically, anthocyanins enhanced the Bcl-2/Bax and caspase-dependent apoptotic pathways through targeting the PI3K/AKT/survivin pathway, resulting in impairment of growth of CRC.
花色苷是一种具有抗癌作用的天然水溶性糖苷类色素，属于黄酮类化合物，具有良好的生物活性，可有效对抗多种癌症，包括结直肠癌（CRC）。然而，花色苷抗癌的确切分子机制尚不清楚；也不清楚针对结直肠癌使用花色苷的合理剂量是多少。
采用 Western blot、免疫组织化学、MTT 检测、异种移植模型和苏木精-伊红（HE）染色进行实验。
与对照组相比，花色苷可显著抑制人结肠癌 HT29 细胞的活力和增殖，并促进其凋亡。此外，花色苷可降低结直肠肿瘤小鼠模型中的肿瘤重量和体积，并下调 PI3K 蛋白的表达，抑制 AKT 的表达和磷酸化，降低肿瘤组织中 Bcl-2 和 Bax 的比值，减少存活素蛋白的表达。
花色苷可促进 CRC 细胞凋亡，抑制结直肠癌细胞异种移植瘤的生长。其机制可能是通过靶向 PI3K/AKT/存活素通路增强 Bcl-2/Bax 和 caspase 依赖的凋亡途径，从而损害 CRC 的生长。
