Shen D T, Gorham J R, Jones R H, Crawford T B
Am J Vet Res. 1978 May;39(5):875-6.
Laboratory-colonized mosquitoes, Culex tarsalis, aedes aegypti, Culiseta inornata, and Anopheles free-borni, and the biting gnat, Culicoides variipennis, were exposed to equine infectious anemia virus. Exposure to the virus was by intrathoracic inoculation for mosquitoes and by oral ingestion of an infective blood meal through a membrane for C variipennis. After various intervals, groups of 15 to 20 insects were homogenized and inoculated into susceptible ponies. Positive immunodiffusion test results were used as criterion for equine infectious anemia infection in ponies. Virus was not detected in the 4 species of mosquitoes at 3, 6, 12, and 18 days after inoculation, or in C variipennis at 6, 8, 12, 14, 21, and 26 days after oral exposure to the virus.
将实验室饲养的致倦库蚊、埃及伊蚊、无味库蚊、弗里伯恩按蚊以及刺螫库蠓暴露于马传染性贫血病毒。对于蚊子，通过胸腔内接种使其接触病毒；对于刺螫库蠓，则通过膜饲使其口服感染性血餐接触病毒。在不同间隔时间后，将15至20只昆虫的群体匀浆并接种到易感小马体内。阳性免疫扩散试验结果被用作小马感染马传染性贫血的标准。在接种后3、6、12和18天，未在4种蚊子中检测到病毒；在口服接触病毒后6、8、12、14、21和26天，也未在刺螫库蠓中检测到病毒。
