Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
J Infect Public Health. 2020 May;13(5):709-717. doi: 10.1016/j.jiph.2019.11.007. Epub 2019 Dec 9.
Middle East respiratory syndrome coronavirus (MERS-CoV) was primarily detected in 2012 and still causing disease in human and camel. Camel and bats have been identified as a potential source of virus for disease spread to human. Although, significant information related to MERS-CoV disease, spread, infection, epidemiology, clinical features have been published, A little information is available on the sequence diversity of Spike protein gene. The Spike protein gene plays a significant role in virus attachment to host cells. Recently, the information about recombinant MERS-CoV has been published. So, this work was designed to identify the emergence of any another recombinant virus in Jeddah, Saudi Arabia.
In this study samples were collected from both human and camels and the Spike protein gene was amplified and sequenced. The nucleotide and amino acid sequences of MERS-CoV Spike protein gene were used to analyze the recombination, genetic diversity and phylogenetic relationship with selected sequences from Saudi Arabia.
The nucleotide sequence identity ranged from 65.7% to 99.8% among all the samples collected from human and camels from various locations in the Kingdom. The lowest similarity (65.7%) was observed in samples from Madinah and Dammam. The phylogenetic relationship formed different clusters with multiple isolates from various locations. The sample collected from human in Jeddah hospital formed a closed cluster with human samples collected from Buraydah, while camel sample formed a closed cluster with Hufuf isolates. The phylogenetic tree by using Aminoacid sequences formed closed cluster with Dammam, Makkah and Duba isolates. The amino acid sequences variations were observed in 28/35 samples and two unique amino acid sequences variations were observed in all samples analyzed while total 19 nucleotides sequences variations were observed in the Spike protein gene. The minor recombination events were identified in eight different sequences at various hotspots in both human and camel samples using recombination detection programme.
The generated information from this study is very valuable and it will be used to design and develop therapeutic compounds and vaccine to control the MERS-CoV disease spread in not only in the Kingdom but also globally.
中东呼吸综合征冠状病毒（MERS-CoV）于 2012 年首次被发现，至今仍在人类和骆驼中引发疾病。骆驼和蝙蝠已被确定为病毒传播给人类的潜在来源。尽管已经发表了大量与 MERS-CoV 疾病、传播、感染、流行病学和临床特征相关的重要信息，但有关 Spike 蛋白基因序列多样性的信息却很少。Spike 蛋白基因在病毒附着到宿主细胞中起着重要作用。最近，有关重组 MERS-CoV 的信息已经发表。因此，这项工作旨在确定在沙特阿拉伯吉达是否出现任何其他重组病毒。
在这项研究中，从人和骆驼中采集样本，并扩增和测序 Spike 蛋白基因。使用 MERS-CoV Spike 蛋白基因的核苷酸和氨基酸序列分析来自沙特阿拉伯的选定序列的重组、遗传多样性和系统发育关系。
从沙特各地不同地点采集的人和骆驼样本的核苷酸序列同一性在 65.7%至 99.8%之间。在麦地那和达曼采集的样本中观察到最低的相似性（65.7%）。形成不同聚类的系统发育关系与来自不同地点的多个分离株有关。从吉达医院采集的人样本与从布赖代采集的人样本形成了一个封闭的聚类，而骆驼样本与胡富夫的分离株形成了一个封闭的聚类。使用氨基酸序列构建的系统发育树与达曼、麦加和杜巴的分离株形成了封闭的聚类。在分析的所有样本中观察到 28/35 个样本的氨基酸序列变异，并且在所有分析的样本中观察到两个独特的氨基酸序列变异，而 Spike 蛋白基因中观察到 19 个核苷酸序列变异。在人和骆驼样本的不同热点处使用重组检测程序鉴定了 8 个不同序列中的轻微重组事件。
本研究产生的信息非常有价值，它将用于设计和开发治疗化合物和疫苗，以控制不仅在沙特阿拉伯，而且在全球范围内的 MERS-CoV 疾病传播。
Zotero 插件
