Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
Wuhan National Biosafety Laboratory, Mega-Science Center for Bio-Safety Research, Chinese Academy of Sciences, Wuhan, Hubei, China.
Nat Commun. 2019 Dec 12;10(1):5677. doi: 10.1038/s41467-019-13589-1.
An important goal of the Zika virus (ZIKV) vaccine is to prevent a congenital syndrome in fetuses of pregnant women, but studies directly evaluating maternal vaccination for ZIKV are lacking. Here we report maternal vaccination using a live-attenuated ZIKV vaccine (3'UTR-∆10-LAV) in a pregnant mouse model. Maternal immunization with 3'UTR-∆10-LAV does not cause any adverse effects on pregnancy, fetal development, or offspring behavior. One maternal immunization fully protects dams against ZIKV infection and in utero transmission. Although neutralizing antibody alone is sufficient to prevent in utero transmission, a higher neutralizing titer is required to protect pregnant mice against in utero transmission than that required to protect non-pregnant mice against viral infection. The immunized dams transfer maternal antibodies to pups, which protect neonates against ZIKV infection. Notably, pregnancy weakens maternal T cell response to 3'UTR-∆10-LAV vaccination. Our results suggest that, besides vaccinating non-pregnant individuals, 3'UTR-∆10-LAV may also be considered for maternal vaccination.
寨卡病毒（ZIKV）疫苗的一个重要目标是预防孕妇胎儿的先天性综合征，但缺乏直接评估母体接种 ZIKV 疫苗的研究。在这里，我们报告了在怀孕小鼠模型中使用活减毒寨卡病毒疫苗（3'UTR-∆10-LAV）进行的母体疫苗接种。3'UTR-∆10-LAV 的母体免疫接种不会对妊娠、胎儿发育或后代行为造成任何不良影响。一次母体免疫接种就能完全保护母体免受 ZIKV 感染和宫内传播。尽管中和抗体本身足以预防宫内传播，但保护怀孕小鼠免受宫内传播所需的中和抗体滴度高于保护未怀孕小鼠免受病毒感染所需的中和抗体滴度。免疫的母鼠将母体抗体转移给幼崽，从而保护新生儿免受寨卡病毒感染。值得注意的是，妊娠会削弱母体对 3'UTR-∆10-LAV 疫苗接种的 T 细胞反应。我们的研究结果表明，除了为未怀孕个体接种疫苗外，3'UTR-∆10-LAV 也可考虑用于母体疫苗接种。
