废水中及活性污泥中存在对克拉霉素耐药的突变证据。
Evidence of mutations conferring resistance to clarithromycin in wastewater and activated sludge.
作者信息
Gnida Anna, Felis Ewa, Ziembińska-Buczyńska Aleksandra, Łuczkiewicz Aneta, Surmacz-Górska Joanna, Olańczuk-Neyman Krystyna
机构信息
1Department of Environmental Biotechnology, Faculty of Energy and Environmental Engineering, Silesian University of Technology, Akademicka 2A, 44-100 Gliwice, Poland.
2Department of Water and Wastewater Technology, Faculty of Civil and Environmental Engineering, Gdańsk University of Technology, G. Narutowicza 11/12, 80-952 Gdańsk, Poland.
出版信息
3 Biotech. 2020 Jan;10(1):7. doi: 10.1007/s13205-019-1989-9. Epub 2019 Nov 29.
The occurrence of clarithromycin in wastewater samples and of the activated sludge bacteria possibly resistant to this pharmaceutical was the object of the study. Samples of wastewater or activated sludge were taken from a municipal wastewater treatment plant in summer and winter and characterised regarding their clarithromycin concentrations and the presence of nucleic acid fragments (Cla-sequences) known to be responsible for clarithromycin resistance in . The concentrations of clarithromycin in raw wastewater were about 1086-2271 ng/L. Around 50-60% less of the pharmaceutical was found in treated wastewater. The concentrations were much higher in winter samples, as compared to summer samples. The clarithromycin resistance markers in were detected by fluorescence in situ hybridisation in activated sludge bacterial cells. Cla-sequences were found in all the detected , independently of the sampling season. Among nitrifying or phosphate or glycogen accumulating bacteria only spp. revealed presence of the clarithromycin sequences.
本研究的对象是废水样本中克拉霉素的出现情况以及活性污泥细菌中可能对这种药物具有抗性的情况。在夏季和冬季从一个城市污水处理厂采集废水或活性污泥样本，并对其克拉霉素浓度以及已知与克拉霉素抗性有关的核酸片段（Cla序列）的存在情况进行表征。原废水中克拉霉素的浓度约为1086 - 2271纳克/升。处理后的废水中该药物的含量大约少50 - 60%。与夏季样本相比，冬季样本中的浓度要高得多。通过荧光原位杂交在活性污泥细菌细胞中检测到了克拉霉素抗性标记。在所有检测到的[细菌名称未明确给出]中都发现了Cla序列，与采样季节无关。在硝化细菌、聚磷菌或糖原积累菌中，只有[具体细菌名称未明确给出]显示存在克拉霉素序列。
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