Wang Xiang, He Ping, Yi Shengyang, Wang Chundie
1Department of Emergency, Hainan Provincial People's Hospital, No.8 of Longhua Road, Haikou, 570100 Hainan Province China.
2Health Center, Hainan Provincial People's Hospital, Haikou, 570100 Hainan Province China.
3 Biotech. 2019 Dec;9(12):451. doi: 10.1007/s13205-019-1986-z. Epub 2019 Nov 14.
This study was undertaken to investigate the effect of natural bioactive compound thearubigin on neonatal acute lung injury (ALI) using LPS-induced ALI as a model. We also attempted to understand the possible underlying mechanism. The effect of thearubigin on lung wet-to-dry weight ratio, the activity of LDH, lung histopathology, BALF protein levels, the activity of MPO, production and extravasation of cytokines and oxidative stress were studied. The results showed that thearubigin caused a significant reduction in lung inflammation as evident from lung wet-to-dry weight ratio, BALF protein levels and MPO activity and histopathological analysis. It was further observed that the attenuation in inflammation happened due to a significant reduction in cytokine levels in alveolar cavities. Thearubigin also showed strong antioxidant properties as evidenced by reduced levels of oxygen species such as HO, MDA and OH ion. Additionally, the antioxidant response element nuclear factor erythroid-2-related factor 2 (Nrf2) pathway was found to be activated in thearubigin-treated group. These results provided a possible mechanism of antioxidant activity of thearubigin in neonatal ALI. Overall, this study showed that thearubigin can be a natural alternative for the treatment of neonatal ALI. However, further studies are required to understand its mechanism antioxidant and anti-inflammatory action.
本研究旨在以脂多糖诱导的急性肺损伤（ALI）为模型，探讨天然生物活性化合物茶红素对新生儿急性肺损伤的影响。我们还试图了解其可能的潜在机制。研究了茶红素对肺湿重与干重比、乳酸脱氢酶（LDH）活性、肺组织病理学、支气管肺泡灌洗液（BALF）蛋白水平、髓过氧化物酶（MPO）活性、细胞因子产生与渗出以及氧化应激的影响。结果表明，从肺湿重与干重比、BALF蛋白水平、MPO活性及组织病理学分析来看，茶红素可显著减轻肺部炎症。进一步观察发现，炎症减轻是由于肺泡腔中细胞因子水平显著降低所致。茶红素还表现出强大的抗氧化特性，如羟基自由基（HO）、丙二醛（MDA）和氢氧根离子（OH离子）等氧物种水平降低。此外，在茶红素处理组中发现抗氧化反应元件核因子红细胞2相关因子2（Nrf2）通路被激活。这些结果为茶红素在新生儿ALI中的抗氧化活性提供了一种可能的机制。总体而言，本研究表明茶红素可能是治疗新生儿ALI的天然替代品。然而，需要进一步研究以了解其抗氧化和抗炎作用的机制。
