Lu Qinghua, Lu Jun, Ma Hailin, Zhang Hao, Wang Lei
School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, 014010 People's Republic of China.
3 Biotech. 2019 Dec;9(12):452. doi: 10.1007/s13205-019-1977-0. Epub 2019 Nov 14.
Xylanase is widely used in pulp and paper bleaching. In this study, the effects of rare earth ions (La, Ce, Er and Gd) on the activity of xylanase produced by are investigated and the application of a xylanase solution containing La in paper bleaching is presented. Our results indicate that the bleaching effect of the enzyme solution containing La was markedly better when the concentration of La was 10 g/L after 4 days of incubation. The mechanism of lanthanum on the improvement of xylanase activity was revealed through electrical conductivity, atomic absorption spectrometer, infrared spectroscopy and fluorescence microscopy analyses. The PCR result clearly demonstrates that a low concentration of La led to the transversions of three base pair of gene sequences. Our experiment also reveals that the La may have been involved in the cellular metabolic processes of and intervened in the base pairing and DNA replication. This research may provide new insights into the improvement of enzymatic activity by lanthanum (III) and its application in paper pulp bleaching.
木聚糖酶广泛应用于纸浆和纸张漂白。在本研究中,研究了稀土离子(镧、铈、铒和钆)对[具体微生物名称缺失]产生的木聚糖酶活性的影响,并介绍了含镧木聚糖酶溶液在纸张漂白中的应用。我们的结果表明,在孵育4天后,当镧浓度为10 g/L时,含镧酶溶液的漂白效果明显更好。通过电导率、原子吸收光谱仪、红外光谱和荧光显微镜分析揭示了镧提高木聚糖酶活性的机制。PCR结果清楚地表明,低浓度的镧导致基因序列的三个碱基对发生颠换。我们的实验还表明,镧可能参与了[具体微生物名称缺失]的细胞代谢过程,并干预了碱基配对和DNA复制。本研究可能为镧(III)提高酶活性及其在纸浆漂白中的应用提供新的见解。