Nishana Eranthodika, Bhat Sham Subraya, Sahana Kaup Sathish, Hegde Sundeep Kuloor, Bhat Vidya, Kalal Bhuvanesh Sukhlal
Department of Pedodontics, Yenepoya Dental College, Yenepoya (Deemed to be University), Deralakatte, Mangaluru-575018, Karnataka, India.
Department of Pediatrics, Yenepoya Medical College, Yenepoya (Deemed to be University), Mangaluru-575018, Karnataka, India.
Rep Biochem Mol Biol. 2019 Jul;8(2):132-138.
Dental caries is a chronic disease among children and pneumonia is often seen in young children. Soluble CD14 (sCD14) protein is released by monocytes and changes in periodontal infection. The study aimed to estimate the level of salivary sCD14 in children with early childhood caries in association with pneumonia.
This case-control study was conducted on 52 children aged between 2 to 5 years. A total of 17 children who were caries free, with no past systemic illness; 17 children with dental caries with no history of systemic illness or dental treatment for caries, and 18 children with caries and pneumonia were included in the control and test groups respectively. Unstimulated saliva of all children was collected. All samples were tested using a commercial available sCD14 ELISA kit.
The sCD14 level was elevated in all three groups. One-way ANOVA was used to compare the mean level of sCD14 values between the groups. Control group had the highest mean sCD14 values (15070.99 ± 4296.44), followed by the caries group (13629.83 ± 5603.76) and pneumonia group (8566.86 ± 4778.81). There is a significant difference between the groups with p=0.001.
Based on the results of the study, it can be concluded that sCD14 can be used as an indicator of the healthy functioning of the oral cavity.
龋齿是儿童中的一种慢性疾病，肺炎在幼儿中也较为常见。可溶性CD14（sCD14）蛋白由单核细胞释放，在牙周感染时会发生变化。本研究旨在评估患有早期儿童龋齿且伴有肺炎的儿童唾液中sCD14的水平。
本病例对照研究针对52名年龄在2至5岁的儿童进行。对照组包括17名无龋齿且无既往全身性疾病的儿童；试验组分别包括17名患有龋齿但无全身性疾病病史或龋齿治疗史的儿童，以及18名患有龋齿且患有肺炎的儿童。收集了所有儿童的非刺激性唾液。所有样本均使用市售的sCD14 ELISA试剂盒进行检测。
三组儿童的sCD14水平均有所升高。采用单因素方差分析比较各组之间sCD14值的平均水平。对照组的平均sCD14值最高（15070.99±4296.44），其次是龋齿组（13629.83±5603.76）和肺炎组（8566.86±4778.81）。各组之间存在显著差异，p = 0.001。
基于本研究结果，可以得出结论，sCD14可作为口腔健康功能的指标。
