Isaza-Guzmán D M, Aristizábal-Cardona D, Martínez-Pabón M C, Velásquez-Echeverri H, Tobón-Arroyave S I
POPCAD Research Group, Laboratory of Immunodetection and Bioanalysis, Faculty of Dentistry, University of Antioquia, Medellín, Colombia.
Oral Dis. 2008 Jul;14(5):450-6. doi: 10.1111/j.1601-0825.2007.01400.x.
To assess the concentration of soluble CD14 receptor in saliva of people with periodontal disease and healthy patients and its relationship with periodontal status.
Unstimulated whole saliva samples from patients with chronic periodontitis (n = 34), aggressive periodontitis (n = 19) and healthy controls (n = 17) were obtained for the study. The periodontal status of each subject was assessed by criteria based on probing depth, clinical attachment loss and the extent of periodontal breakdown. The levels of sCD14 were measured in saliva samples with an enzyme-linked immunosorbent assay (ELISA).
Although no significant difference (P > 0.05) was found for salivary sCD14 levels between periodontitis groups, they were significantly greater (P < 0.05) than those detected for healthy controls. Furthermore, Spearman's correlation analysis showed statistically significant correlations (P < 0.01) between data from salivary sCD14 levels and clinical measurements.
The findings of the present study reemphasize the importance of whole saliva as sampling method in terms of immunological purposes in periodontal disease and suggest that the elevated sCD14 concentration may be one of the host-response components associated with the clinical manifestations of periodontal disease.
评估牙周病患者和健康受试者唾液中可溶性CD14受体的浓度及其与牙周状况的关系。
采集慢性牙周炎患者（n = 34）、侵袭性牙周炎患者（n = 19）和健康对照者（n = 17）的非刺激性全唾液样本进行研究。根据探诊深度、临床附着丧失和牙周破坏程度的标准评估每个受试者的牙周状况。采用酶联免疫吸附测定（ELISA）法检测唾液样本中sCD14的水平。
虽然牙周炎组之间唾液sCD14水平无显著差异（P > 0.05），但均显著高于健康对照组（P < 0.05）。此外，Spearman相关分析显示，唾液sCD14水平数据与临床测量数据之间存在统计学显著相关性（P < 0.01）。
本研究结果再次强调了全唾液作为牙周病免疫研究采样方法的重要性，并表明sCD14浓度升高可能是与牙周病临床表现相关的宿主反应成分之一。
