Prester Ljerka, Protrka Nives, Macan Jelena, Katunarić Marina
1Institute for Medical Research and Occupational Health, Ksaverska cesta 2, HR-10001 Zagreb, Croatia.
2Private dental practice, Zagreb, Croatia.
Arh Hig Rada Toksikol. 2017 Dec 20;68(4):315-321. doi: 10.1515/aiht-2017-68-2974.
CD14 is a co-receptor involved in the recognition of Gram-negative and Gram-positive bacteria, the latter known to cause dental caries. The aim of this study was to determine whether soluble CD14 (sCD14) in saliva was associated with caries activity and the collection method from the saliva. The study included 55 participants aged 20 to 40 years, 30 with dental caries and 25 caries-free controls. We collected 110 saliva samples in total, 55 of resting saliva and 55 of mechanically stimulated saliva. Median levels of sCD14, measured with a matrix-matched enzyme-linked immunosorbent assay (ELISA), were higher in the caries-active than in the caries-free group in either resting (203.3 vs.167.9 ng mL-1; P<0.01) or stimulated saliva (201 vs.105.7 ng mL-1; P<0.01). The resting salivary flow rate was lower in the caries-active than caries-free group (0.61±0.42 vs. 0.98±0.52 mL min-1; P<0.01). Hyposalivation was observed only in the caries-active group (10 and 13 % in stimulated and resting saliva, respectively). Higher salivary sCD14 levels and secretion rates were clearly associated with dental caries and resting saliva. Future studies should focus on the clinical utility of salivary sCD14 as a potential biomarker and predictor of future caries events.
CD14是一种共受体，参与革兰氏阴性菌和革兰氏阳性菌的识别，后者已知可导致龋齿。本研究的目的是确定唾液中的可溶性CD14（sCD14）是否与龋齿活动以及唾液采集方法有关。该研究纳入了55名年龄在20至40岁之间的参与者，其中30人患有龋齿，25人无龋齿作为对照。我们总共收集了110份唾液样本，55份静息唾液样本和55份机械刺激唾液样本。用基质匹配酶联免疫吸附测定（ELISA）测得的sCD14中位数水平，无论是静息唾液（203.3对167.9 ng/mL；P<0.01）还是刺激唾液（201对105.7 ng/mL；P<0.01），龋齿活跃组均高于无龋齿组。龋齿活跃组的静息唾液流速低于无龋齿组（0.61±0.42对0.98±0.52 mL/min；P<0.01）。仅在龋齿活跃组观察到唾液分泌减少（刺激唾液和静息唾液中分别为10%和13%）。唾液sCD14水平和分泌率较高显然与龋齿和静息唾液有关。未来的研究应关注唾液sCD14作为潜在生物标志物和未来龋齿事件预测指标的临床应用。
