Leibniz Institute of Photonic Technology (Leibniz IPHT) Jena, Member of the Leibniz Research Alliance-Leibniz Health Technologies, Albert-Einstein-Straße 9, 07745, Jena, Germany.
BioSolutions Halle GmbH, Weinbergweg 22, 06120, Halle (Saale), Germany.
Appl Microbiol Biotechnol. 2020 Jan;104(1):405-415. doi: 10.1007/s00253-019-10286-3. Epub 2019 Dec 12.
Recently Legionella pneumophila is the main causative waterborne organism of severe respiratory infections. Additionally, other Legionella species are documented as human pathogens. In our work, we describe a rapid detection method which combines two advantages for sensitive and specific detection of the genus Legionella: the fast isothermal amplification method "Loop-mediated isothermal AMPlification" (LAMP), and a colorimetric detection method using the metal indicator hydroxynaphtol blue (HBN) which allows to determine an optical signal with a simple readout (with the naked eye). Moreover, we present two approaches for minimizing the assay volume using a stationary microchip LAMP and droplet digital-based LAMP (ddLAMP) as promising highly sensitive setups.
最近嗜肺军团菌是严重呼吸道感染的主要水源性病原体。此外，其他军团菌物种也被记录为人病原体。在我们的工作中，我们描述了一种快速检测方法，该方法结合了两种优势，用于灵敏和特异性检测军团菌属：快速等温扩增方法“环介导等温扩增”（LAMP），以及使用金属指示剂羟基萘酚蓝（HBN）的比色检测方法，该方法允许用简单的读数（肉眼）来确定光学信号。此外，我们提出了两种方法来最小化使用固定微芯片 LAMP 和液滴数字 LAMP（ddLAMP）的测定体积，这两种方法都是很有前途的高灵敏度设置。
