Department of Oral Medicine, Faculdade de Odontologia, Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227 - Butantã, São Paulo, SP, 05508-000, Brazil.
College of Dentistry, University of Saskatchewan, Saskatoon, SK, Canada.
Clin Oral Investig. 2020 Feb;24(2):559-568. doi: 10.1007/s00784-019-03165-8. Epub 2019 Dec 12.
To present a systematic review that provides updated information about proteins found in salivary fluid extracted strictly from ducts.
The systematic review probing strategy was based on electronic databases word search (PubMed, EMBASE, LILACS, Web of Science, and Scopus). Risk of bias was assessed based on Joanna Briggs Institute Critical Appraisal Checklist for Studies Reporting Prevalence Data.
After 2 rounds of scrutiny, 12 articles were included, totaling 231 individuals (125 were healthy, 41 were elder individuals with radicular caries, 56 had primary Sjögren's syndrome, and 9 were patients who had received radiotherapy for head and neck cancer). The selected studies had no similarities among proteins found, demonstrating the need of standard reference in experimental methodology to obtain a thorough coverage of proteins.
Further studies are required to better determine the relative amount of proteins described in this study. It is essential to increase the number of samples, to perform similar collection techniques, to include other analyses methods such as mass spectrometry, and to perform the validation of some proteins using immunoassay techniques such as Elisa and Western blot.
Proteomic profile of saliva collected from ducts is essential to better understand the disease process, enabling the identification of biomarkers for specific clinical situations.
系统回顾提供了关于严格从导管中提取的唾液液中发现的蛋白质的最新信息。
系统综述探测策略基于电子数据库的单词搜索（PubMed、EMBASE、LILACS、Web of Science 和 Scopus）。基于 Joanna Briggs 研究所流行数据报告的批判性评估清单评估偏倚风险。
经过两轮审查，共纳入 12 篇文章，总计 231 人（125 人为健康人，41 人为有根龋的老年患者，56 人为原发性干燥综合征患者，9 人为头颈部癌症接受放疗的患者）。所选研究中发现的蛋白质没有相似之处，这表明需要标准的实验方法参考，以全面涵盖蛋白质。
需要进一步研究以更好地确定本研究中描述的蛋白质的相对含量。增加样本数量、进行类似的采集技术、纳入其他分析方法（如质谱）以及使用免疫测定技术（如 ELISA 和 Western blot）对一些蛋白质进行验证至关重要。
从导管中收集的唾液的蛋白质组学特征对于更好地了解疾病过程至关重要，能够识别特定临床情况的生物标志物。
