College of Environmental Science and Engineering, Taiyuan University of Technology, No. 209 of University Street, Yuci District, Jinzhong, 030600, Shanxi, China.
College of Biomedical Engineering, Taiyuan University of Technology, No. 209 of University Street, Yuci District, Jinzhong, 030600, Shanxi, China.
Curr Microbiol. 2020 Mar;77(3):491-499. doi: 10.1007/s00284-019-01833-2. Epub 2019 Dec 12.
Bacillus sp. TYF-LIM-B05, which is isolated from spoilage vinegar, is resistant to high temperature, high concentrated alcohol, acid, and salt, and can produce ethanol from mono-, di-, polysaccharide, and complex biomass as the sole carbon source. Thus, this strain is a potential candidate for consolidated bioprocessing (CBP) of fermenting lignocellulose to ethanol in a single step. To provide insight into the key enzymes involved in lignocellulose degradation and ethanol production, a draft genome of TYF-LIM-B05 was developed in this study. The results indicated that 348 genes are related to carbohydrate transport and metabolism according to the clusters of orthologous groups of proteins and annotated 187 CAZy domains from a total of 61 different families. The presence of genes encoding laccases, quinone oxidoreductases/reductases, and aryl-alcohol dehydrogenases further implies that TYF-LIM-B05 has the potential to degrade lignin. Remarkably, this strain has the ability to catalyze the oxidative decarboxylation of pyruvate to acetyl-CoA by pyruvate dehydrogenase complexes. The genomic information provided in this study will help researchers to better understand the mechanisms of the lignocellulose degradation and ethanol production pathway in thermophiles.
从变质醋中分离到的芽孢杆菌 TYF-LIM-B05 具有耐高温、高浓度酒精、酸和盐的抗性，能够将单糖、二糖、多糖和复杂生物质作为唯一碳源转化为乙醇。因此，该菌株是木质纤维素一步发酵生产乙醇的整合生物加工（CBP）的潜在候选菌株。为了深入了解木质纤维素降解和乙醇生产中涉及的关键酶，本研究对 TYF-LIM-B05 的基因组草图进行了开发。结果表明，根据蛋白质直系同源簇（COG）和总共 61 个不同家族的 187 个 CAZy 结构域注释，有 348 个基因与碳水化合物的运输和代谢有关。存在编码漆酶、醌氧化还原酶/还原酶和芳醇脱氢酶的基因进一步表明，TYF-LIM-B05 具有降解木质素的潜力。值得注意的是，该菌株具有通过丙酮酸脱氢酶复合物催化丙酮酸氧化脱羧生成乙酰辅酶 A 的能力。本研究提供的基因组信息将有助于研究人员更好地了解嗜热菌中木质纤维素降解和乙醇生产途径的机制。
