Tan Yizhou, Zhang Min, Wu Zhifeng, Chen Jingqin, Ren Yaguang, Liu Chengbo, Gu Ying
Department of Laser Medicine, the First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.
Laser Medicine Center, Hainan Hospital, Chinese PLA General Hospital, Sanya 572013, China.
Photoacoustics. 2025 May 17;44:100730. doi: 10.1016/j.pacs.2025.100730. eCollection 2025 Aug.
Current consensus suggests a simultaneous occurrence of hypoxia and inflammation. For the first time, we observed a hyperoxia state during the initiation stage of gouty arthritis (GA) via optical-resolution photoacoustic microscopy. GA as a paradigm of acute sterile inflammation, has been regarded as a single process. However, our experimental results demonstrated that the onset-resolution inflammation process composed of two sub-processes with different features. In the initial sub-process, inflammation and resolution events appear in hyperoxia state (1st-5th days). In the subsequent sub-process, post-resolution events appear in hypoxia state (6th-15th days), which is related with the second wave of immune response. Furthermore, we demonstrated that the inflammatory cytokines together with hyperoxia levels in initial sub-process can be downregulated by photobiomodulation, resulting in the hypoxia levels in subsequent sub-process were inhibited. Our results unveiled the detailed progress of GA and provided potential non-invasive monitoring and treatment strategies.
目前的共识认为缺氧和炎症是同时发生的。我们首次通过光学分辨率光声显微镜在痛风性关节炎（GA）的起始阶段观察到高氧状态。GA作为急性无菌性炎症的范例，一直被视为一个单一的过程。然而，我们的实验结果表明，发病-消退炎症过程由两个具有不同特征的子过程组成。在初始子过程中，炎症和消退事件出现在高氧状态（第1-5天）。在随后的子过程中，消退后事件出现在缺氧状态（第6-15天），这与第二波免疫反应有关。此外，我们证明了在初始子过程中，光生物调节可以下调炎症细胞因子和高氧水平，从而抑制随后子过程中的缺氧水平。我们的结果揭示了GA的详细进展，并提供了潜在的非侵入性监测和治疗策略。
