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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Rothstein M, Coppens M

Division of Cell and Molecular Biology, State University of New York, Buffalo 14214.

Comp Biochem Physiol B. 1978;61(1):99-104. doi: 10.1016/0305-0491(78)90222-5.

Soy-peptone has been fractionated to yield a series of increasingly purified components which sharply increase the populations of Caenorhabditis briggsae and Caenorhabditis elegans when added to the basal medium. The nutritionally active material appears to be a small polypeptide. 2. C. briggsae and C. elegans routinely reach populations of 150,000/ml or greater in 9 days in still culture, starting from an inoculum of only 500 organisms per ml. C. elegans is particularly sensitive to the depth of the medium. However, large populations can be achieved in deep cultures if continuous shaking is carried out. 3. Panagrellus silusiae shows improved populations if the basal medium is supplemented with the nutritional factor from soy-peptone. However, 0.5% acetic acid or 1% ethanol added to the medium serves equally well. There is no additive effect of ethanol and the factor.

大豆蛋白胨已被分级分离，以产生一系列纯度不断提高的组分，当将这些组分添加到基础培养基中时，能显著增加布氏秀丽隐杆线虫和秀丽隐杆线虫的种群数量。营养活性物质似乎是一种小肽。2. 在静止培养中，从每毫升仅500个生物体的接种量开始，布氏秀丽隐杆线虫和秀丽隐杆线虫通常在9天内达到每毫升150,000个或更多的种群数量。秀丽隐杆线虫对培养基的深度特别敏感。然而，如果进行连续振荡，在深层培养中也可以获得大量种群。3. 如果基础培养基添加大豆蛋白胨中的营养因子，嗜菌新小杆线虫的种群数量会增加。然而，向培养基中添加0.5%的乙酸或1%的乙醇同样有效。乙醇和该因子没有相加作用。

Rothstein M, Coppens M

Division of Cell and Molecular Biology, State University of New York, Buffalo 14214.

Comp Biochem Physiol B. 1978;61(1):99-104. doi: 10.1016/0305-0491(78)90222-5.

Soy-peptone has been fractionated to yield a series of increasingly purified components which sharply increase the populations of Caenorhabditis briggsae and Caenorhabditis elegans when added to the basal medium. The nutritionally active material appears to be a small polypeptide. 2. C. briggsae and C. elegans routinely reach populations of 150,000/ml or greater in 9 days in still culture, starting from an inoculum of only 500 organisms per ml. C. elegans is particularly sensitive to the depth of the medium. However, large populations can be achieved in deep cultures if continuous shaking is carried out. 3. Panagrellus silusiae shows improved populations if the basal medium is supplemented with the nutritional factor from soy-peptone. However, 0.5% acetic acid or 1% ethanol added to the medium serves equally well. There is no additive effect of ethanol and the factor.

大豆蛋白胨已被分级分离，以产生一系列纯度不断提高的组分，当将这些组分添加到基础培养基中时，能显著增加布氏秀丽隐杆线虫和秀丽隐杆线虫的种群数量。营养活性物质似乎是一种小肽。2. 在静止培养中，从每毫升仅500个生物体的接种量开始，布氏秀丽隐杆线虫和秀丽隐杆线虫通常在9天内达到每毫升150,000个或更多的种群数量。秀丽隐杆线虫对培养基的深度特别敏感。然而，如果进行连续振荡，在深层培养中也可以获得大量种群。3. 如果基础培养基添加大豆蛋白胨中的营养因子，嗜菌新小杆线虫的种群数量会增加。然而，向培养基中添加0.5%的乙酸或1%的乙醇同样有效。乙醇和该因子没有相加作用。