Vanfleteren J R
Experientia. 1976 Aug 15;32(8):1087-8. doi: 10.1007/BF01933985.
A method is presented for the large scale cultivation of the free-living nematode Caenorhabditis elegans, using continuous aeration and agitation in glass ware (stirrer flasks) developed for the continuous culture of suspended cells. With this technique, populations up to 10(9) nematodes may be obtained in a 10 1 culture in less than 6 weeks with an inoculum of some 50 worms. Costs can be reduced by using an inexpensive yeast extract, available from the food industry.
本文介绍了一种大规模培养自由生活线虫秀丽隐杆线虫的方法，该方法使用专为悬浮细胞连续培养而开发的玻璃器皿（搅拌瓶）进行连续通气和搅拌。采用这种技术，接种约50条线虫，在不到6周的时间内，在10升培养物中可获得多达10⁹条线虫。使用食品工业提供的廉价酵母提取物可降低成本。
