DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands.
Delft University of Technology, Department of Biotechnology, van der Maasweg 9, 2629 HZ Delft, The Netherlands.
Curr Opin Biotechnol. 2020 Apr;62:189-195. doi: 10.1016/j.copbio.2019.11.012. Epub 2019 Dec 13.
Downstream processing aims at recovering the target product at the required specifications from the bioreactor effluent. Research and development in this field relies on experimental and mathematical tools at the levels of chemical components, unit operations and processes. Recently, advances have been made in addressing the broth mixture complexity early on, in incorporating high-throughput experimentation for data generation and mechanistic understanding of the separation processes, in improving the materials and scalability of specific unit operations, as well as establishing the potential of process integration concepts. Further developments are expected considering the variety of (non-sugar) feedstocks currently under research, the need to transition to renewable energy sources, and the opportunities for improved scale-up through initiatives as Big Data and digital manufacturing.
下游处理旨在从生物反应器流出物中按所需规格回收目标产物。该领域的研究和开发依赖于化学组分、单元操作和过程层面的实验和数学工具。最近，在早期解决发酵液混合物复杂性、采用高通量实验生成数据以及对分离过程的机理理解、改进特定单元操作的材料和可扩展性，以及确定过程集成概念的潜力方面都取得了进展。考虑到目前正在研究的各种（非糖）原料、向可再生能源过渡的需求，以及通过大数据和数字制造等举措提高放大规模的机会，预计还会有进一步的发展。
