Department of Forensic Entomology, Institute of Legal Medicine at the University Hospital Jena, 07740, Jena, Germany.
Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Sci Rep. 2019 Dec 16;9(1):19151. doi: 10.1038/s41598-019-55127-5.
The flesh fly genus Wohlfahrtia Brauer & Bergenstamm contains at least six species of medical and veterinary importance. Traditional methods of species identification in specimens of Wohlfahrtia, however, are restricted mostly to adult forms. Muscle attachment site (MAS) patterns allow for species determination in larval forms. MAS patterns in third instar larvae of six common West Palearctic species of Wohlfahrtia have been analyzed for this study. As in previously investigated Calliphoridae and Sarcophagidae, MAS patterns were found to be species specific. A genus pattern was established to be used as base for comparison in further species determination. For the first time a tool is provided for species identification of such broad range in larvae of Wohlfahrtia species. Wohlfahrtia patterns are composed of a significantly higher number of MAS than patterns found in Sarcophaga. Specifics of the six species analyzed are explained in detail. The larvae of the well-known species W. magnifica, an obligate traumatic myiasis agent, had to be excluded from the analysis as a great number of spines on the outside obscure muscle attachment sites on the inside of the cuticle.
肉蝇属 Wohlfahrtia Brauer & Bergenstamm 至少包含六种具有医学和兽医重要性的物种。然而，传统的 Wohlfahrtia 标本物种鉴定方法主要局限于成虫形式。肌附着位点 (MAS) 模式可用于幼虫形式的物种鉴定。本研究分析了 6 种常见的西古北界 Wohlfahrtia 种的第三龄幼虫的 MAS 模式。与先前研究的 Calliphoridae 和 Sarcophagidae 一样，MAS 模式被发现具有物种特异性。建立了一个属模式作为进一步物种鉴定的比较基础。这是首次为 Wohlfahrtia 属幼虫提供了如此广泛的物种鉴定工具。Wohlfahrtia 模式由明显多于在 Sarcophaga 中发现的 MAS 组成。详细解释了分析的 6 种物种的具体情况。由于大量的刺在外面掩盖了角质层内部的肌附着点，因此必须排除众所周知的创伤性蝇蛆病病原体 W. magnifica 的幼虫进行分析。
