Torres-Martínez Yuridia, Arredondo-Espinoza Eder, Puente Carlos, González-Santiago Omar, Pineda-Aguilar Nayely, Balderas-Rentería Isaías, López Israel, Ramírez-Cabrera Mónica A
Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Laboratorio de Farmacología Molecular y Modelos Biológicos, Monterrey, Nuevo León, Mexico.
Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Laboratorio de Materiales I, San Nicolás de los Garza, Nuevo León, Mexico.
PeerJ. 2019 Dec 9;7:e8142. doi: 10.7717/peerj.8142. eCollection 2019.
In this study, silver nanoparticles (NP) were synthesized by two methods: using an aqueous extract of leaves and using citrate ions as stabilizing agent, and the cytotoxicity and anticancer activity of both NP were evaluated in vitro. The particles synthesized with the aqueous extract were spherical with a size ranging from 15 to 45 nm. These NP decreased cell viability in all of the cells studied; however, the IC could only be estimated in the Chang liver cells (IC = 21.37 µg/mL). These particles also decreased the generation of reactive oxygen species in Chang and SiHa cells. Additionally, the dispersions decreased the activity of caspase-3. There was no significant difference between the biological activities of the NP obtained with the aqueous extract and the NP synthesized using citrate ions. This study showed that an aqueous extract of is an excellent alternative for the synthesis of silver NP. These NP showed cytotoxicity and anticancer activity in vitro. Although more experiments are required, the cell death occurs probably through a mechanism different from apoptosis.
在本研究中，通过两种方法合成了银纳米颗粒（NP）：使用树叶水提取物并以柠檬酸根离子作为稳定剂，并且在体外评估了两种NP的细胞毒性和抗癌活性。用水提取物合成的颗粒呈球形，尺寸范围为15至45纳米。这些NP降低了所有研究细胞中的细胞活力；然而，仅能在Chang肝细胞中估计IC（IC = 21.37 µg/mL）。这些颗粒还减少了Chang和SiHa细胞中活性氧的产生。此外，分散体降低了caspase-3的活性。用水提取物获得的NP与使用柠檬酸根离子合成的NP的生物活性之间没有显著差异。本研究表明，树叶水提取物是合成银NP的极佳替代物。这些NP在体外显示出细胞毒性和抗癌活性。尽管需要更多实验，但细胞死亡可能通过不同于凋亡的机制发生。
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